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Cellular imaging
From Lisa
Contacts
Olivier DEBEIR - Christine DECAESTECKER - Ivan ADANJA - Philippe VAN HAM
Project description
A complete device was developed by the SLN/LISA laboratory in collaboration with the Laboratory of Toxicology of the ULB Faculty of Pharmacy. A cluster of such devices were installed at this latter laboratory where they run 24h per day for monitoring cancer cell behavior and providing a large number of video sequences.
The aim of this device is to permit the observation during several days of in vitro cell cultures by means of phase-contrast microscopy, in order to automatically detect and quantify specific behavior patterns, such as cell motility, cell division, cell death...
Specific image acquisition and analysis software packages have been developed for different applications using standard 2D cell cultures. New developments are in course for the analysis of cell behavior in 3D matrix gels. This project is supported by the F.R.M.S. and the Télévie program from the F.N.R.S. (Belgian National Fund for Scientific Research).
This application is a good example of the vertical integration of a complete system from the assisted video microscipy and image analysis system at the service of the state of the art in cancer research.
The Cellular Imaging project is :
- incubator prototyping
- thermo-regulation
- image acquisition
- image analysis
- motion parameters estimation
- division parameters estimation
- cancer cell migration study
- anti-cancer drug screening
|  Thermoregulated incubator |
Downloads
Example of a tracking achieved on a phase contrast video sequence : Media:Zoom_xVid.avi (xVid codec)
High-resolution celltracking sequence [1]
Mitosis tracking detail [2]
Pie-shaped multi-meanshift tracking details [3]
Large population tracking [4]
Mitosis detection [5]
Related publications
Adanja, I., Megalizzi, V., Debeir, O., Decaestecker, C., A new method to address unmet needs for extracting individual cell migration features from a large number of cells embedded in 3D volumesPLoS ONE, Vol. 6, pp. e22263, 2011 BibtexBibtex : pmid21789244
Ivan Adanja, Olivier Debeir, Véronique Mégalizzi, Robert Kiss, Nadine Warzée, Christine Decaestecker, Automated tracking of unmarked cells migrating in three-dimensional matrices applied to anti-cancer drug screeningExperimental Cell Research, Vol. 316, 2, pp. 181 - 193, 2010 BibtexBibtex : adanja10
Leyman, S., Sidani, M., Ritsma, L., Waterschoot, D., Eddy, R., Dewitte, D., Debeir, O., Decaestecker, C., Vandekerckhove, J., van Rheenen, J., Ampe, C., Condeelis, J., Van Troys, M., Unbalancing the PI(4,5)P2-Cofilin Interaction Impairs Cell SteeringMol. Biol. Cell, Vol. 20, pp. 4509-23, Nov 2009 BibtexBibtex : leyman09
Megalizzi, V., Decaestecker, C., Debeir, O., Spiegl-Kreinecker, S., Berger, W., Lefranc, F., Kast, R. E., Kiss, R., Screening of anti-glioma effects induced by sigma-1 receptor ligands: Potential new use for old anti-psychiatric medicinesEur. J. Cancer, Vol. 45, pp. 2893-905, Nov 2009 BibtexBibtex : mégalizzi09
Abstract : The prognosis of glioblastoma (GBM) remains poor. Diffuse invasion of distant brain tissue by migrating cells from the primary tumour mass has already occurred at time of diagnosis. Anti-cancer effects of a selective sigma-1 agonist, 4-(N-benzylpiperidin-4-yl)-4-iodobenzamide (4-IBP), in glioblastoma were shown previously, leading to the present work where the effects on glioblastoma cells of 17 agonists or antagonists of sigma-1 receptors were studied, including currently marketed drugs fluvoxamine, dextromethorphan, donepezil, memantine and haloperidol. We first showed that established GBM cell lines, primary cultures and surgical specimens express sigma-1 receptors. In vitro analyses then focused on anti-proliferation and anti-migratory effects on human glioblastoma cell lines using quantitative videomicroscopy analyses. These cell monitoring assays revealed specific impacts on the mitotic cell process. Using an aggressive glioma model orthotopically grafted into the brains of immunocompromised mice, we showed that combining donepezil and temozolomide gave additive benefit in terms of long survivors as compared to temozolomide or donepezil alone. Clinical study is planned if further rodent dose-ranging studies of donepezil with temozolomide continue to show evidence of benefit in this model.
S. Rorive, A. Berton, N. D'haene, C. N. Takacs, O. Debeir, C. Decaestecker, I. Salmon, Matrix metalloproteinase-9 interplays with the IGFBP2-IGFII complex to promote cell growth and motility in astrocytomas.Glia, Vol. 56, 15, pp. 1679-90, 2008 BibtexBibtex : pmid658242
Debeir O., Adanja I., Kiss R., Decaestecker C., Models of cancer cell migration and cellular imaging and analysisThe Motile Actin System in Health and Disease, pp. 123-156, Kerala (India), 2008 BibtexBibtex : debeir08b
O. Debeir, I. Adanja, N. Warzée, P. Van Ham, C. Decaestecker, Phase contrast image segmentation by weak watershed transform assemblyProceedings of 5th IEEE International Symposium on Biomedical Imaging : From Nano to Macro (ISBI), pp. 724-727, 2008 BibtexBibtex : debeir08d
Debeir O., Mégalizzi V., Warzée N., Kiss R., Decaestecker C., Videomicroscopic extraction of specific information on cell proliferation and migration in vitroExp. Cell Res., Vol. 314, pp. 2985-2998, Oct 2008 BibtexBibtex : debeir08
Mijatovic, T., Gailly, P., Mathieu, V., De Nève, N., Yeaton, P., Kiss, R., Decaestecker, C., Neurotensin is a versatile modulator of in vitro human pancreatic ductal adenocarcinoma cell (PDAC) migrationCell. Oncol., Vol. 29, pp. 315--326, 2007 BibtexBibtex : mijatovic07
Abstract : BACKGROUND: While the neurotensin (NT) roles in pancreatic cancer growth are well documented, its effects on pancreatic cancer cell migration have not been described. METHODS: The NT-induced effects on the migration process of human pancreatic ductal adenocarcinoma cells (PDACs) were characterized by means of various assays including computer-assisted video-microscopy, fluorescence microscopy, ELISA-based, small GTPase pull-down and phosphorylation assays. RESULTS: The NT-induced modifications on in vitro PDACs migration largely depended on the extra-cellular matrix environment and cell propensity to migrate collectively or individually. While NT significantly reduced the level of migration of collectively migrating PDACs on vitronectin, it significantly increased the level of individually migrating PDACs. These effects were mainly mediated through the sortilin/NTR3 receptor. Neurotensin both induced altered expression of alphaV and beta5 integrin subunits in PDACs cultured on vitronectin resulting in modified adhesion abilities, and caused modifications to the organization of the actin cytoskeleton through the NT-mediated activation of small Rho GTPases. While the NT effects on individually migrating PDACs were mediated at least through the EGFR/ERK signaling pathways, those on collectively migrating PDACs appeared highly dependent on the PI 3-kinase pathway. CONCLUSION: This study strongly suggests the involvement of neurotensin in the modulation of human PDAC migration.
Mégalizzi V., Mathieu V., Mijatovic T., Gailly P., Debeir O., De Neve N., Van Damme M., Bontempi G., Haibe-Kains B., Decaestecker C., Kondo Y., Kiss R., Lefranc F., 4-IBP, a sigma1 receptor agonist, decreases the migration of human cancer cells, including glioblastoma cells, in vitro and sensitizes them in vitro and in vivo to cytotoxic insults of proapoptotic and proautophagic drugs.Neoplasia, Vol. 9, 5, pp. 358-69, May 2007 BibtexBibtex : megalizzi07
Decaestecker C., Debeir O., Van Ham P., Kiss R., Can anti-migratory drugs be screened in vitro? A review of 2D and 3D assays for the quantitative analysis of cell migration.Med Res Rev, Vol. 27, 2, pp. 149-76, March 2007 BibtexBibtex : decaestecker07r
Servotte S., Camby I., Debeir O., Deroanne C., Lambert C. A., Lapière C. M, Kiss R., Nusgens B., Decaestecker C., The In Vitro Influences of Neurotensin on the Motility Characteristics of Human U373 Glioblastoma Cells.Neuropathology and Applied Neurobiology, Vol. 32, 6, pp. 575-84, Dec 2006 BibtexBibtex : servotte05
Note : accepted for publication 2005
C. Hayot, O. Debeir, P. Van Ham, M. Van Damme, R. Kiss, C. Decaestecker, Characterization of the activities of actin-affecting drugs on tumor cell migration.Toxicology and Applied Pharmacology, Vol. 211, 1, pp. 30-40, Feb 2006 BibtexBibtex : hayot06
O. Debeir, P. Van Ham, R. Kiss, C. Decaestecker, Tracking of Migrating Cells Under Phase-Contrast Video Microscopy With Combined Mean-Shift ProcessesIEEE Transaction on Medical Imaging, Vol. 24, 6, pp. 697-711, June 2005 BibtexBibtex : debeir05
F. Lefranc, T. Mijatovic, V. Mathieu, S. Rorive, C. Decaestecker, O. Debeir, J. Brotchi, P. Van Ham, I. Salmon, R. Kiss, Characterization of gastrin-induced proangiogenic effects in vivo in orthotopic u373 experimental human glioblastomas, and in vitro in human umbilical vein endothelial cells (huvec)Clin Cancer Res, Vol. 10, 24, pp. 8250-65, Dec 2004 BibtexBibtex : lefranc04
O. Debeir, I. Camby, R. Kiss, P. Van Ham, C. Decaestecker, A Model-Based Approach For Automated In Vitro Cell Tracking And Chemotaxis AnalysesCytometry, Vol. 60A, 1, pp. 29-40, July 2004 BibtexBibtex : debeir04
Belot N, Pochet R, Heizmann C-W, Kiss R, Decaestecker C, Extracellular S100A4 stimulates the migration rate of astrocytic tumor cells by modifying the organization of their actin cytoskeletonBiochim Biophys Acta, Vol. 1600, 1-2, pp. 74--83, Nov 2002 BibtexBibtex : belot02
Nagy N, Brenner C, Markadieu N, Chaboteaux C, Camby I, Schafer B W, Pochet R, Heizmann C W, Salmon I, Kiss R, Decaestecker C, S100A2, a putative tumor suppressor gene, regulates in vitro squamous cell carcinoma migrationLab Invest, Vol. 81, 4, pp. 599--612, Apr 2001 BibtexBibtex : nagy01
De Hauwer, C., Darro, F., Camby, I., Kiss, R., Van Ham, P., Decaestecker, C., In vitro motility evaluation of aggregated cancer cells by means of automatic image processingCytometry, Vol. 36, pp. 1--10, May 1999 BibtexBibtex : de hauwer99
Farinelle S, Dehauwer C, Darro F, Decaestecker C, Fontaine J, Pasteels JL, Van Ham P, Atassi G, Kiss R, Setting up of an original computer-assisted methodology to characterize in vitro drug-induced anti-angiogenic effectsInt J Mol Med, Vol. 2, 5, pp. 545--553, Nov 1998 BibtexBibtex : farinelle98
Note : JOURNAL ARTICLE
De Hauwer, C, Camby, I, Darro, F, Migeotte, I, Decaestecker, C, Verbeek, C, Danguy, A, Pasteels, J L, Brotchi, J, Salmon, I, Van Ham, P, Kiss, R, Gastrin inhibits motility, decreases cell death levels and increases proliferation in human glioblastoma cell linesJ Neurobiol, Vol. 37, 3, pp. 373--382, Nov 1998 BibtexBibtex : de98
De Hauwer, C, Camby, I, Darro, F, Decaestecker, C, Gras, T, Salmon, I, Kiss, R, Van Ham, P, Dynamic characterization of glioblastoma cell motilityBiochem Biophys Res Commun, Vol. 232, 2, pp. 267--272, March 1997 BibtexBibtex : de97
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