Christine DECAESTECKERFrom Lisa
ContactcdecaesATulb.ac.be ULB - LIST - LISA CP165/57 Teaching
Research topicsImage and data analysis applied to:
PublicationsPapersFerdinande, L., Decaestecker, C., Verset, L., Mathieu, A., Moles Lopez, X., Negulescu, A. M., Van Maerken, T., Salmon, I., Cuvelier, C. A., Demetter, P.,Clinicopathological significance of indoleamine 2,3-dioxygenase 1 expression in colorectal cancer Br. J. Cancer, Vol. 106, pp. 141--147, Jan 2012 Bibtex Bibtex : pmid22108515 Tondeleir, Davina, Lambrechts, Ania, Mueller, Mathias, Jonckheere, Veronique, Doll, Thierry, Vandamme, Drieke, Bakkali, Karima, Waterschoot, Davy, Lemaistre, Marianne, Debeir, Olivier, Decaestecker, Christine, Hinz, Boris, Staes, An, Timmerman, Evy, Colaert, Niklaas, Gevaert, Kris, Vanderkerckove, Joel, Ampe, Christophe, Cells lacking β-actin are genetically reprogrammed and maintain conditional migratory capacity Molecular & cellular proteomics, Vol. 11, 8, pp. 255-71, 2012 Bibtex Bibtex : info:hdl:2013/113768 Note : DOI: 10.1074/mcp.M111.015099 Moles Lopez, Xavier, Debeir, Olivier, Maris, Calliope, Rorive, Sandrine, Roland, Isabelle, Saerens, Marco, Salmon, Isabelle, Decaestecker, Christine, Clustering methods applied in the detection of Ki67 hot-spots in whole tumor slide images: An efficient way to characterize heterogeneous tissue-based biomarkers. Cytometry. Part A, Vol. 81, 9, pp. 765-775, 2012 Bibtex Bibtex : info:hdl:2013/127791 Note : DOI: 10.1002/cyto.a.22085 Abstract : Whole-slide scanners allow the digitization of an entire histological slide at very high resolution. This new acquisition technique opens a wide range of possibilities for addressing challenging image analysis problems, including the identification of tissue-based biomarkers. In this study, we use whole-slide scanner technology for imaging the proliferating activity patterns in tumor slides based on Ki67 immunohistochemistry. Faced with large images, pathologists require tools that can help them identify tumor regions that exhibit high proliferating activity, called "hot-spots" (HSs). Pathologists need tools that can quantitatively characterize these HS patterns. To respond to this clinical need, the present study investigates various clustering methods with the aim of identifying Ki67 HSs in whole tumor slide images. This task requires a method capable of identifying an unknown number of clusters, which may be highly variable in terms of shape, size, and density. We developed a hybrid clustering method, referred to as Seedlink. Compared to manual HS selections by three pathologists, we show that Seedlink provides an efficient way of detecting Ki67 HSs and improves the agreement among pathologists when identifying HSs. © 2012 International Society for Advancement of Cytometry. Duray, A., Descamps, G., Arafa, M., Decaestecker, C., Remmelink, M., Sirtaine, N., Ernoux-Neufcoeur, P., Mutijima, E., Somja, J., Depuydt, C. E., Delvenne, P., Saussez, S., High incidence of high-risk HPV in benign and malignant lesions of the larynx Int. J. Oncol., Vol. 39, pp. 51-59, July 2011 Bibtex Bibtex : pmid21573490 Remmelink, M., de Leval, L., Decaestecker, C., Duray, A., Crompot, E., Sirtaine, N., Andre, S., Kaltner, H., Leroy, X., Gabius, H. J., Saussez, S., Quantitative immunohistochemical fingerprinting of adhesion/growth-regulatory galectins in salivary gland tumours: divergent profiles with diagnostic potential Histopathology, Vol. 58, pp. 543-556, March 2011 Bibtex Bibtex : pmid21410745 Ernoux-Neufcoeur, P., Arafa, M., Decaestecker, C., Duray, A., Remmelink, M., Leroy, X., Herfs, M., Somja, J., Depuydt, C. E., Delvenne, P., Saussez, S., Combined analysis of HPV DNA, p16, p21 and p53 to predict prognosis in patients with stage IV hypopharyngeal carcinoma J. Cancer Res. Clin. Oncol., Vol. 137, pp. 173-181, Jan 2011 Bibtex Bibtex : pmid20376678 Adanja, I., Megalizzi, V., Debeir, O., Decaestecker, C., A new method to address unmet needs for extracting individual cell migration features from a large number of cells embedded in 3D volumes PLoS ONE, Vol. 6, pp. e22263, 2011 Bibtex Bibtex : pmid21789244 Rorive, S., Moles Lopez, X., Maris, C., Trepant, A. L., Sauvage, S., Sadeghi, N., Roland, I., Decaestecker, C., Salmon, I., TIMP-4 and CD63: new prognostic biomarkers in human astrocytomas Mod. Pathol., Vol. 23, pp. 1418-1428, Oct 2010 Bibtex Bibtex : pmid20693981 Balde, E. S., Megalizzi, V., Traore, M. S., Cos, P., Maes, L., Decaestecker, C., Pieters, L., Balde, A. M., In vitro antiprotozoal, antimicrobial and antitumor activity of Pavetta crassipes K. Schum leaf extracts J Ethnopharmacol, Vol. 130, pp. 529-535, Aug 2010 Bibtex Bibtex : pmid20561931 Megalizzi, V., Le Mercier, M., Decaestecker, C., Sigma receptors and their ligands in cancer biology: overview and new perspectives for cancer therapy Med Res Rev, Vol. doi:10.1002/med.20218, Aug 2010 Bibtex Bibtex : pmid20687157 Mathieu, A., Weynand, B., Verbeken, E., Da Silva, S., Decaestecker, C., Salmon, I., Demetter, P., Comparison of four antibodies for immunohistochemical evaluation of epidermal growth factor receptor expression in non-small cell lung cancer Lung Cancer, Vol. 69, pp. 46-50, July 2010 Bibtex Bibtex : pmid19796839 Rorive, S., D'Haene, N., Fossion, C., Delpierre, I., Abarguia, N., Avni, F., Decaestecker, C., Salmon, I., Ultrasound-guided fine-needle aspiration of thyroid nodules: stratification of malignancy risk using follicular proliferation grading, clinical and ultrasonographic features Eur. J. Endocrinol., Vol. 162, pp. 1107-1115, June 2010 Bibtex Bibtex : pmid20219856 Saussez, S., de Leval, L., Decaestecker, C., Sirtaine, N., Cludts, S., Duray, A., Chevalier, D., Andre, S., Gabius, H. J., Remmelink, M., Leroy, X., Galectin fingerprinting in Warthin's tumors: lectin-based approach to trace its origin? Histol. Histopathol., Vol. 25, pp. 541-550, May 2010 Bibtex Bibtex : pmid20238293 Pozdzik, A. A., Berton, A., Schmeiser, H. H., Missoum, W., Decaestecker, C., Salmon, I. J., Vanherweghem, J. L., Nortier, J. L., Aristolochic acid nephropathy revisited: a place for innate and adaptive immunity? Histopathology, Vol. 56, pp. 449-463, March 2010 Bibtex Bibtex : pmid20459552 Le Mercier, M., Lamoral-Theys, D., Le Calve, B., Rynkowski, M. A., Bruyere, C., Decaestecker, C., Haibe-Kains, B., Bontempi, G., Dubois, J., Lefranc, F., Kiss, R., Long-term temozolomide treatment induces marked amino metabolism modifications and an increase in TMZ sensitivity in Hs683 oligodendroglioma cells Neoplasia, Vol. 12, pp. 69-79, Jan 2010 Bibtex Bibtex : pmid20072655 Lamoral-Theys, D., Decaestecker, C., Mathieu, V., Dubois, J., Kornienko, A., Kiss, R., Evidente, A., Pottier, L., Lycorine and its derivatives for anticancer drug design} Mini Rev Med Chem, Vol. 10, pp. 41-50, Jan 2010 Bibtex Bibtex : pmid20105122 Ivan Adanja, Olivier Debeir, Véronique Mégalizzi, Robert Kiss, Nadine Warzée, Christine Decaestecker, Automated tracking of unmarked cells migrating in three-dimensional matrices applied to anti-cancer drug screening Experimental Cell Research, Vol. 316, 2, pp. 181 - 193, 2010 Bibtex Bibtex : adanja10 Wauthoz N., Deleuze Ph., Hecq J., Roland I., Saussez S., Adanja I., Debeir O., Decaestecker C., Mathieu V., Kiss R., Amighi K., In Vivo Assessment of Temozolomide Local Delivery for Lung Cancer Inhalation Therapy European Journal of Pharmaceutical Sciences, Vol. 39, pp. 402-411, 2010 Bibtex Bibtex : wauthoz10a Le Calve, B., Rynkowski, M., Le Mercier, M., Bruyere, C., Lonez, C., Gras, T., Haibe-Kains, B., Bontempi, G., Decaestecker, C., Ruysschaert, J. M., Kiss, R., Lefranc, F., Long-term in vitro treatment of human glioblastoma cells with temozolomide increases resistance in vivo through up-regulation of GLUT transporter and aldo-keto reductase enzyme AKR1C expression Neoplasia, Vol. 12, pp. 727-739, 2010 Bibtex Bibtex : pmid20824049 Megalizzi, V., Decaestecker, Debeir, O., Spiegl-Kreinecker, S., Berger, W., Lefranc, F., Kast, R. E., Kiss, R., Screening of anti-glioma effects induced by sigma-1 receptor ligands: Potential new use for old anti-psychiatric medicines Eur. J. Cancer, Vol. 45, pp. 2893-905, Nov 2009 Bibtex Bibtex : mégalizzi09 Abstract : The prognosis of glioblastoma (GBM) remains poor. Diffuse invasion of distant brain tissue by migrating cells from the primary tumour mass has already occurred at time of diagnosis. Anti-cancer effects of a selective sigma-1 agonist, 4-(N-benzylpiperidin-4-yl)-4-iodobenzamide (4-IBP), in glioblastoma were shown previously, leading to the present work where the effects on glioblastoma cells of 17 agonists or antagonists of sigma-1 receptors were studied, including currently marketed drugs fluvoxamine, dextromethorphan, donepezil, memantine and haloperidol. We first showed that established GBM cell lines, primary cultures and surgical specimens express sigma-1 receptors. In vitro analyses then focused on anti-proliferation and anti-migratory effects on human glioblastoma cell lines using quantitative videomicroscopy analyses. These cell monitoring assays revealed specific impacts on the mitotic cell process. Using an aggressive glioma model orthotopically grafted into the brains of immunocompromised mice, we showed that combining donepezil and temozolomide gave additive benefit in terms of long survivors as compared to temozolomide or donepezil alone. Clinical study is planned if further rodent dose-ranging studies of donepezil with temozolomide continue to show evidence of benefit in this model. Leyman, S., Sidani, M., Ritsma, L., Waterschoot, D., Eddy, R., Dewitte, D., Debeir, O., Decaestecker, C., Vandekerckhove, J., van Rheenen, J., Ampe, C., Condeelis, J., Van Troys, M., Unbalancing the PI(4,5)P2-Cofilin Interaction Impairs Cell Steering Mol. Biol. Cell, Vol. 20, pp. 4509-23, Nov 2009 Bibtex Bibtex : leyman09 Decaestecker, C., Moles Lopez, X., D'Haene, N., Roland, I., Guendouz, S., Duponchelle, C., Berton, A., Debeir, O., Salmon, I., Requirements for the valid quantification of immunostains on tissue microarray materials using image analysis Proteomics, Vol. 8, pp. 1739--1750, Aug 2009 Bibtex Bibtex : decaes09 Abstract : Antibody-based proteomics applied to tissue microarray (TMA) technology provides a very efficient means of visualizing and locating antigen expression in large collections of normal and pathological tissue samples. To characterize antigen expression on TMAs, the use of image analysis methods avoids the effects of human subjectivity evidenced in manual microscopical analysis. Thus, these methods have the potential to significantly enhance both precision and reproducibility. Although some commercial systems include tools for the quantitative evaluation of immunohistochemistry-stained images, there exists no clear agreement on best practices to allow for correct and reproducible quantification results. Our study focuses on practical aspects regarding (i) image acquisition (ii) segmentation of staining and counterstaining areas and (iii) extraction of quantitative features. We illustrate our findings using a commercial system to quantify different immunohistochemistry markers targeting proteins with different expression patterns (cytoplasmic, nuclear or membranous) in colon cancer or brain tumor TMAs. Our investigations led us to identify several steps that we consider essential for standardizing computer-assisted immunostaining quantification experiments. In addition, we propose a data normalization process based on reference materials to be able to compare measurements between studies involving different TMAs. In conclusion, we recommend certain critical prerequisites that commercial or in-house systems should satisfy in order to permit valid immunostaining quantification. Capouillez, A., Debauve, G., Decaestecker, C., Filleul, O., Chevalier, D., Mortuaire, G., Coppée, F., Leroy, X., Belayew, A., Saussez, S., The helicase-like transcription factor is a strong predictor of recurrence in hypopharyngeal but not in laryngeal squamous cell carcinomas Histopathology, Vol. 55, pp. 77--90, July 2009 Bibtex Bibtex : capouillez09 Abstract : AIMS: To examine the immunohistochemical expression of helicase-like transcription factor (HLTF) in relation to the prognosis of hypopharyngeal (HSCCs) and laryngeal (LSCCs) squamous cell carcinomas, and to characterize the HLTF protein variants expressed in biopsy specimens of head and neck squamous cell carcinoma (HNSCC) as well as the HeLa cell line. METHODS AND RESULTS: HLTF expression was determined by immunohistochemistry on a series of 100 hypopharyngeal (stage IV) and 56 laryngeal SCCs (stages I, II and IV). The HLTF variants were defined using reverse transcriptase-polymerase chain reaction and Western blots in 13 fresh HNSCC biopsies and in HeLa cells. High levels of HLTF expression were associated with rapid recurrence rates in a subgroup of 81 stage IV hypopharyngeal SCCs (with complete follow-up). A 95-kDa HLTF variant truncated at the carboxyl-terminal domain was detected in addition to the 115-kDa full-size protein in HNSCC biopsies, while six variants were observed in HeLa cells. CONCLUSIONS: Our results demonstrate, for the first time, that hypopharyngeal SCCs presenting high levels of HLTF have a worse prognosis. The quantitative determination of HLTF in hypopharyngeal SCCs was an independent prognostic marker alongside tumour node metastasis staging. HNSCCs expressed the truncated HLTF variant lacking the domains involved in DNA repair. Le Mercier M., Fortin S., Mathieu V., Roland I., Spiegl-Kreinecker S., Haibe-Kains B., Bontempi G., Decaestecker C., Berger W., Lefranc F., Kiss R., Galectin 1 proangiogenic and promigratory effects in the Hs683 oligodendroglioma model are partly mediated through the control of BEX2 expression Neoplasia, Vol. 11, pp. 485-496, May 2009 Bibtex Bibtex : pmid19412433 Abstract : We have previously reported that galectin 1 (Gal-1) plays important biological roles in astroglial as well as in oligodendroglial cancer cells. As an oligodendroglioma model, we make use of the Hs683 cell line that has been previously extensively characterized at cell biology, molecular biology, and genetic levels. Galectin 1 has been shown to be involved in Hs683 oligodendroglioma chemoresistance, neoangiogenesis, and migration. Down-regulating Gal-1 expression in Hs683 cells through targeted small interfering RNA provokes a marked decrease in the expression of the brain-expressed X-linked gene: BEX2. Accordingly, the potential role of BEX2 in Hs683 oligodendroglioma cell biology has been investigated. The data presented here reveal that decreasing BEX2 expression in Hs683 cells increases the survival of Hs683 orthotopic xenograft-bearing mice. Furthermore, this decrease in BEX2 expression impairs vasculogenic mimicry channel formation in vitro and angiogenesis in vivo, and modulates glioma cell adhesion and invasive features through the modification of several genes previously reported to play a role in cancer cell migration, including MAP2, plexin C1, SWAP70, and integrin beta(6). We thus conclude that BEX2 is implicated in oligodendroglioma biology. Maréchal R., Demetter P., Nagy N., Berton A., Decaestecker C., Polus M., Closset J., Devière J., Salmon I., Van Laethem J. L., High expression of CXCR4 may predict poor survival in resected pancreatic adenocarcinoma Br. J. Cancer, Vol. 100, pp. 1444-1451, May 2009 Bibtex Bibtex : maréchal09 Abstract : Chemokines and their receptors are involved in tumourigenicity and clinicopathological significance of chemokines receptor expression in pancreatic adenocarcinoma (PA) is not fully understood. This study was conducted to determine patients' outcome according to the expressions of CXCR4, CXCR7 and HIF-1alpha after resection of PA. Immunohistochemistry for CXCR4, CXCR7 and HIF-1alpha expressions as well as cell proliferative index (Ki-67) was conducted in 71 resected (R0) PA and their 48 related lymph nodes (LN) using tissue microarray. CXCR4 and CXCR7 expressions were positively correlated to HIF-1alpha suggesting a potential role of HIF-1alpha in CXCR4 and CXCR7 transcription activation. Patients with CXCR4(high) tumour expression had shorter OS than those with low expression (median survival: 9.7 vs 43.2 months, P=0.0006), a higher risk of LN metastases and liver recurrence. In multivariate analysis, high CXCR4 expression, LN metastases and poorly differentiated tumour are independent negative prognosis factors. In a combining analysis, patients with CXCR4(low)/CXCR7(low) tumour had a significantly shorter DFS and OS than patients with a CXCR7(high)/CXCR4(high) tumour. CXCR4 in resected PA may represent a valuable prognostic factor as well as an attractive target for therapeutic purpose. Saussez S., Javadian R., Hupin C., Magremanne M., Chantrain G., Loeb I., Decaestecker C., Bisphosphonate-related osteonecrosis of the jaw and its associated risk factors: a Belgian case series Laryngoscope, Vol. 119, pp. 323-329, Feb 2009 Bibtex Bibtex : pmid321451521 Abstract : OBJECTIVES: Bisphosphonate-related osteonecrosis of the jaw (BROJ) is a serious oral complication of bisphosphonate (BP) treatment involving the exposure of necrotic maxillary or mandibular bone. Our purpose is to describe the clinical presentation of 34 cases of BROJ and to identify potential risk factors. STUDY DESIGN: A retrospective study was performed in four Belgian institutions. METHODS: Complete medical histories were recorded and analyzed. These data include age, gender, initial disease requiring BP, type and duration of BP treatment, symptomatology and location of BROJ, prior dental procedures, treatment of the BROJ and treatment outcome, and radiographic, histological, and microbiological data. RESULTS: Bisphosphonates (BP) were used in the management of disseminated cancers in 30 patients (88.5% of total studied), while four patients received BP due to osteoporosis (11.5%). The most frequently used BP was zoledronic acid in 29 patients (83%). Microbiological data obtained in 25 patients demonstrated that 72% of these patients were infected or colonized by an actinomyces. Eight of the 14 patients (57%) who received only medical treatment were cured. Of the 20 patients who underwent surgical treatments, only four were completely cured (20%). BROJ lesions smaller than 1 cm are associated with better prognosis in terms of treatment outcomes (P = .0009). Local treatments combined with long-term antibiotics are also correlated with better prognosis (P = .02). CONCLUSIONS: Lesions smaller than 1 cm and lesions that were subject to medical treatments are associated with a better outcome. Surgical treatments appear to be non-beneficial for BROJ. Saussez S., Cludts S., Capouillez A., Mortuaire G., Smetana K., Kaltner H., André S., Leroy X., Gabius H. J., Decaestecker C., Identification of matrix metalloproteinase-9 as an independent prognostic marker in laryngeal and hypopharyngeal cancer with opposite correlations to adhesion/growth-regulatory galectins-1 and -7 Int. J. Oncol., Vol. 34, pp. 433-439, Feb 2009 Bibtex Bibtex : pmid19148478 Abstract : The enzymatic activity of matrix metalloproteinase-9 (MMP-9) suggests that its presence in hypopharyngeal and laryngeal squamous cell carcinomas (HSCCs, LSCCs) could have prognostic value. We tested this hypothesis by quantitative morphometric analysis of immunohistochemical staining in histological sections of 73 stage IV HSCCs and 45 LSCCs (30 cases of stage I/II, 15 cases of stage IV). As compared to tumour-free epithelium an increase for the labelling index in LSCCs reached statistical significance (p=0.04). Specimens of Reinke's edema were strongly higher in this parameter compared to tumour-free tissue area (p=0.000001), underscoring an association between the level of MMP-9 expression and inflammation. Focusing on patients' recurrence status we identified thresholds for the labelling index of 10% for HSCCs and 18% for LSCCs, both indicating rapid recurrence and dismal prognosis unless surpassed. When relating data for MMP-9 to those for three adhesion/growth-regulatory galectins, a positive correlation with galectin-7 expression was detected in LSCCs. This finding suggests a possible potential role of this endogenous lectin as inducer of MMP-9 gene expression in situ. Of note, galectin-1 expression was negatively correlated with MMP-9 and that of galectin-3, a substrate of MMP-9, not related. In conclusion our study delineated a prognostic role of MMP-9 immunodetection in high-stage HSCCs and in LSCCs when separating patients by a distinct threshold for the labelling index. Moreover, it indicated associations between MMP-9 and multifunctional galectins-1 and -7 in situ. Saussez S., Decaestecker C., Cludts S., Ernoux P., Chevalier D., Smetana K., André S., Leroy X., Gabius H. J., Adhesion/growth-regulatory tissue lectin galectin-1 in relation to angiogenesis/lymphocyte infiltration and prognostic relevance of stromal up-regulation in laryngeal carcinomas Anticancer Res., Vol. 29, pp. 59-65, Jan 2009 Bibtex Bibtex : pmid19331133 Abstract : BACKGROUND: Galectin-1 has been found to modulate lymphocyte invasion in inflammation and to be involved in angiogenesis in models, thus prompting examination of its clinical relevance in laryngeal cancer. PATIENTS AND METHODS: Immunohistochemical processing of tissue sections (n=53) from patients with stage I/II (n=35) and stage IV (n=18) laryngeal squamous cell carcinoma (LSCC) with a specific anti-galectin-1 antibody and monitoring of CD45/CD31 positivity was combined with quantitative morphometric analysis. RESULTS: Lectin presence in the tumor and endothelial cells was positively correlated, while a negative relationship to the number of CD45-positive lymphocytes was demonstrated. No association was seen with the extent of neovascularization. The mean optical density (MOD) of lectin-dependent staining in the tumor stroma was significantly increased compared to normal stroma. CONCLUSION: Galectin-1 was not associated with angiogenesis in the studied cohort while galectin-1 in endothelial cells may negatively influence lymphocyte invasion and the mean optical density for the stromal galectin-1 signal is up-regulated in tumors. Mathieu A., Nagy N., Decaestecker C., Ferdinande L., Vandenbroucke K., Rottiers P., Cuvelier C. A., Salmon I., Demetter P., Expression of galectins-1, -3 and -4 varies with strain and type of experimental colitis in mice Int J Exp Pathol, Vol. 89, pp. 438-446, Dec 2008 Bibtex Bibtex : amathieu08 Abstract : Galectins are increasingly the focus of biomedical research. Although they are involved at different stages in inflammation, data on galectins in colitis remain scarce. The aim of this study was to determine and compare the expression of galectins in acute and chronic experimental colitis in mice. Immunohistochemistry for galectins-1, -3 and -4 was performed on colon tissue from C57BL/6 and BALB/c mice with acute dextran sodium sulphate colitis and from 129 Sv/Ev IL-10 knock-out (IL-10(-/-)) mice. From these three mouse strains, we first detected major differences in galectin expression related to the genetic background in the control animals. With regard to inflammation, chronic colitis in IL-10(-/-) mice was associated with increased galectin-4 expression; in contrast with the two other models, no galectin-1 and -3 alterations were observed in IL-10(-/-) mice. Acute colitis in C57BL/6 and BALB/c mice showed increased galectin-3 expression in the lamina propria and the crypt epithelium, together with a decreased nuclear expression. These results suggest an involvement of galectins in the development and perpetuation of colonic inflammation and illustrate that the choice of the mouse strain for studying galectins might influence the outcome of the experiments. Capouillez A., Decaestecker C., Filleul O., Chevalier D., Coppée F., Leroy X., Belayew A., Saussez S., Helicase-like transcription factor exhibits increased expression and altered intracellular distribution during tumor progression in hypopharyngeal and laryngeal squamous cell carcinomas Virchows Arch., Vol. 453, pp. 491-499, Nov 2008 Bibtex Bibtex : pmid18825407 Abstract : The helicase-like transcription factor (HLTF) belongs to the SWI/SNF family of proteins that use the energy from adenosine triphosphate hydrolysis to remodel chromatin during a variety of cellular processes. HLTF is also involved in DNA repair. Using computer-assisted microscopy, the immunohistochemical expression of HLTF was determined using a series of 100 hypopharyngeal and 56 laryngeal squamous cell carcinomas (SCCs) compared to tumor-free epithelia (60 cases) and dysplasias (92 cases). In hypopharyngeal SCC tumor progression, increased HLTF expression was associated with the percentage of immunopositive epithelial tissue areas (p = 0.02) and the staining intensity of the positive area (p = 0.0005). In the cases of laryngeal lesions, the immunolabeling intensity of HLTF significantly decreased with malignancy (p = 0.01). We also observed a significant shift of HLTF expression from the cytoplasm toward the nuclear compartment (p = 0.0007). Our data reveal an association between the presence of HLTF and neoplastic progression of hypopharyngeal and laryngeal SCCs. Debeir O., Mégalizzi V., Warzée N., Kiss R., Decaestecker C., Videomicroscopic extraction of specific information on cell proliferation and migration in vitro Exp. Cell Res., Vol. 314, pp. 2985-2998, Oct 2008 Bibtex Bibtex : debeir08 Dumont P., Berton A., Nagy N., Sandras F., Tinton S., Demetter P., Mascart F., Allaoui A., Decaestecker C., Salmon I., Expression of galectin-3 in the tumor immune response in colon cancer Lab. Invest., Vol. 88, pp. 896-906, Aug 2008 Bibtex Bibtex : pmid18542048 Abstract : The role of tumor-associated macrophages (TAMs) is controversial. Although most studies on different cancer types associate them with a poorer prognosis, interestingly in colon cancer, most articles indicate that TAMs prevent tumor development; patients with high TAMs have better prognosis and survival rate. M1-polarized macrophages produce high level of tumor necrosis factor-alpha, interleukin-1 beta or reactive oxygen species, which can effectively kill susceptible tumor cells. In contrast, M2-polarized macrophages can secrete different factors that promote tumor cell growth and survival or favor angiogenesis and tissue invasion. Considering the beneficial role of TAMs in colon cancer, we speculated that they may not display the M2 polarization commonly observed in tumor microenvironment, but rather develop M1 properties. Therefore, we used an in vitro model to analyze the effects of supernatants from M1-polarized macrophages on DLD-1 colon cancer cells. Our data indicate that the conditioned medium from LPS-activated macrophages (CM-LAM) contains a high level of granulocyte-macrophage colony-stimulating factor, interleukins-1 beta, -6, -8 and tumor necrosis factor-alpha, and that it exerts a marked growth inhibitory activity on DLD-1 cells. Prolonged exposure to CM-LAM results in cell death by apoptosis. Such exposure to CM-LAM leads to the modulation of gal-3 expression: we observed a marked downregulation of gal-3 mRNA and protein expression following CM-LAM treatment. We also describe that the knockdown of gal-3 sensitizes DLD-1 cells to CM-LAM. These data suggest an involvement of gal-3 in the response of colon cancer cells to proinflammatory stimuli, such as the conditioned medium from activated macrophages. Le Mercier M., Lefranc F., Mijatovic T., Debeir O., Haibe-Kains B., Bontempi G., Decaestecker C., Kiss R., Mathieu V., Evidence of galectin-1 involvement in glioma chemoresistance Toxicol Appl Pharmacol, Vol. 229, 2, pp. 172-183, June 2008 Bibtex Bibtex : lemercier08b Mijatovic T., Mahieu T., Bruyère C., De Nève N., Dewelle J., Simon G., Dehoux M. J., Van der Aar E., Haibe-Kains B., Bontempi G., Decaestecker C., Van Quaquebeke E., Darro F., Kiss R., UNBS5162, a novel naphthalimide that decreases CXCL chemokine expression in experimental prostate cancers} Neoplasia, Vol. 10, pp. 573-586, June 2008 Bibtex Bibtex : pmid8494561541 Abstract : Several naphthalimides have been evaluated clinically as potential anticancer agents. UNBS3157, a naphthalimide that belongs to the same class as amonafide, was designed to avoid the specific activating metabolism that induces amonafide's hematotoxicity. The current study shows that UNBS3157 rapidly and irreversibly hydrolyzes to UNBS5162 without generating amonafide. In vivo UNBS5162 after repeat administration significantly increased survival in orthotopic human prostate cancer models. Results obtained by the National Cancer Institute (NCI) using UNBS3157 and UNBS5162 against the NCI 60 cell line panel did not show a correlation with any other compound present in the NCI database, including amonafide, thereby suggesting a unique mechanism of action for these two novel naphthalimides. Affymetrix genome-wide microarray analysis and enzyme-linked immunosorbent assay revealed that in vitro exposure of PC-3 cells to UNBS5162 (1 microM for 5 successive days) dramatically decreased the expression of the proangiogenic CXCL chemokines. Histopathology additionally revealed antiangiogenic properties in vivo for UNBS5162 in the orthotopic PC-3 model. In conclusion, the present study reveals UNBS5162 to be a pan-antagonist of CXCL chemokine expression, with the compound displaying antitumor effects in experimental models of human refractory prostate cancer when administered alone and found to enhance the activity of taxol when coadministered with the taxoid. Pozdzik, A. A., Salmon, I. J., Debelle, F. D., Decaestecker, C., Van den Branden, C., Verbeelen, D., Deschodt-Lanckman, M. M., Vanherweghem, J. L., Nortier, J. L., Aristolochic acid induces proximal tubule apoptosis and epithelial to mesenchymal transformation Kidney Int., Vol. 73, pp. 595--607, March 2008 Bibtex Bibtex : pozdzik08a Abstract : Aristolochic acid contamination in herbal remedies leads to interstitial fibrosis, tubular atrophy, and renal failure in humans. To study the cellular mechanisms contributing to the pathophysiology of this renal disease, we studied Wistar rats treated with aristolochic acid and measured tubular and interstitial cell proliferation, epithelial/mesenchymal cell marker expression, tubular membrane integrity, myofibroblast accumulation, oxidative stress, mitochondrial damage, tubular apoptosis, and fibrosis. Oxidative stress, a loss of cadherin concomitant with vimentin expression, basement membrane denudation with active caspase-3 expression, and mitochondrial injury within tubular cells were evident within 5 days of administration of the toxin. During the chronic phase, interstitial mesenchymal cells accumulated in areas of collagen deposits. Impaired regeneration and apoptosis of proximal tubular cells resulted in tubule atrophy with a near absence of dedifferentiated cell transmembrane migration. We suggest that resident fibroblast activation plays a critical role in the process of renal fibrosis during aristolochic acid toxicity. Sadeghi N., D'Haene N., Decaestecker C., Levivier M., Metens T., Maris C., Wikler D., Baleriaux D., Salmon I., Goldman S., Apparent Diffusion Coefficient and Cerebral Blood Volume in Brain Gliomas: Relation to Tumor Cell Density and Tumor Microvessel Density Based on Stereotactic Biopsies AJNR Am J Neuroradiol, Vol. 29, pp. 476-482, 2008 Bibtex Bibtex : sadeghi08 D'Haene N., Catteau X., Maris C., Martin B., Salmon I., Decaestecker C., Endothelial hyperplasia and endothelial galectin-3 expression are prognostic factors in primary central nervous system lymphomas Br J Haematol, Vol. 140, pp. 402-410, 2008 Bibtex Bibtex : d'haene08 Saussez S., Decaestecker C., Mahillon V., Cludts S., Capouillez A., Chevalier D., Vet H. K., André S., Toubeau G., Leroy X., Gabius H. J., Galectin-3 upregulation during tumor progression in head and neck cancer Laryngoscope, Vol. 118, pp. 1583-1590, 2008 Bibtex Bibtex : pmid18677282 Abstract : 10(-6)). CONCLUSIONS: Our data reveal an association between level of presence of galectin-3 and neoplastic progression of HSCCs and LSCCs. Saussez S., Decaestecker C., Lorfevre F., Chevalier D., Mortuaire G., Kaltner H., André S., Toubeau G., Gabius H. J., Leroy X., Increased expression and altered intracellular distribution of adhesion/growth-regulatory lectins galectins-1 and -7 during tumour progression in hypopharyngeal and laryngeal squamous cell carcinomas Histopathology, Vol. 52, 4, pp. 483-493, 2008 Bibtex Bibtex : saussez08b Le Mercier M., Mathieu V., Haibe-Kains B., Bontempi G., Mijatovic T., Decaestecker C., Kiss R., Lefranc F., Knocking Down Galectin 1 in Human Hs683 Glioblastoma Cells Impairs Both Angiogenesis and Endoplasmic Reticulum Stress Responses. Journal of neuropathology and experimental neurology, Vol. 67, 5, pp. 456-469, 2008 Bibtex Bibtex : mercier08 Abstract : Galectin (Gal) 1 is a hypoxia-regulated proangiogenic factor that also directly participates in glioblastoma cell migration. To determine how Gal-1 exerts its proangiogenic effects, we investigated Gal-1 signaling in the human Hs683 glioblastoma cell line. Galectin 1 signals through the endoplasmic reticulum transmembrane kinase/ribonuclease inositol-requiring 1alpha, which regulates the expression of oxygen-regulated protein 150. Oxygen-regulated protein 150 controls vascular endothelial growth factor maturation. Galectin 1 also modulates the expression of 7 other hypoxia-related genes (i.e. CTGF, ATF3, PPP1R15A, HSPA5, TRA1, and CYR61) that are implicated in angiogenesis. Decreasing Gal-1 expression in Hs683 orthotopic xenografts in mouse brains by siRNA administration impaired endoplasmic reticulum stress and enhanced the therapeutic benefits of the proautophagic drug temozolomide. These results suggest that decreasing Gal-1 expression (e.g. through brain delivery of nonviral infusions of anti-Gal-1 siRNA in patients) can represent an additional therapeutic strategy for glioblastoma. Lemy A., Wissing K. M., Rorive S., Zlotta A., Roumeguere T., Muniz Martinez M.-C., Decaestecker C., Salmon I., Abramowicz D., Vanherweghem J.-L., Nortier J., Late onset of bladder urothelial carcinoma after kidney transplantation for end-stage aristolochic acid nephropathy: a case series with 15-year follow-up. American journal of kidney diseases, Vol. 51, 3, pp. 471-477, 2008 Bibtex Bibtex : lemy08 Abstract : BACKGROUND: Aristolochic acids are nephrotoxins and predispose to upper-tract urothelial carcinoma. The risk of bladder urothelial carcinoma after kidney transplantation and its relationship to upper-tract urothelial carcinoma is not well defined. STUDY DESIGN: Case series. SETTING & PARTICIPANTS: Single-center cohort of 38 women given kidney transplants for end-stage aristolochic acid nephropathy. OUTCOMES & MEASUREMENTS: The prevalence of upper urinary tract urothelial carcinoma was determined by collecting pathological results of specimens obtained by means of bilateral ureteronephrectomy. We also established the cumulative incidence of bladder urothelial carcinoma in biopsies performed during prospective screening cystoscopies during a 15-year follow-up. RESULTS: Upper-tract urothelial carcinoma was found in 17 patients with aristolochic acid nephropathy (44.7char37). During follow-up, bladder urothelial carcinoma was diagnosed in 15 patients 68 to 169 months after cessation of aristolochic acid exposure (39.5char37): 8 urothelial carcinoma in situ, 4 noninvasive low-grade papillary urothelial carcinoma, and 3 infiltrating urothelial carcinoma. 12 of 17 patients (71char37) with a history of upper-tract urothelial carcinoma developed bladder urothelial carcinoma during follow-up, whereas this occurred in only 3 of 21 patients (14char37) without upper-tract urothelial carcinoma (P < 0.01). Despite local and/or systemic chemotherapy, 3 patients died and 2 radical cystectomies were performed. LIMITATIONS: Small sample size of this case series. CONCLUSIONS: Upper-tract and bladder urothelial carcinoma are dramatic complications in kidney transplant recipients with aristolochic acid nephropathy, confirming the carcinogenic properties of aristolochic acids. We identified upper-tract urothelial carcinoma as a potent risk factor for the subsequent development of bladder urothelial carcinoma after kidney transplantation for aristolochic acid nephropathy. Because this complication may occur years after aristolochic acid discontinuation, we suggest regular cystoscopies in addition to the bilateral ureteronephrectomy in kidney transplant recipients with aristolochic acid nephropathy. S. Rorive, A. Berton, N. D'haene, C. N. Takacs, O. Debeir, C. Decaestecker, I. Salmon, Matrix metalloproteinase-9 interplays with the IGFBP2-IGFII complex to promote cell growth and motility in astrocytomas. Glia, Vol. 56, 15, pp. 1679-90, 2008 Bibtex Bibtex : pmid658242 Mijatovic T., De Nève N., Gailly P., Mathieu V., Haibe-Kains B., Bontempi G., Lapeira J., Decaestecker C., Facchini V., Kiss R., Nucleolus and c-Myc: potential targets of cardenolide-mediated antitumor activity Mol Cancer Ther, Vol. 7, 5, pp. 1285-1296, 2008 Bibtex Bibtex : mijatovic08 Pozdzik A., Salmon I., Husson C., Decaestecker C., Rogier E., Bourgeade M. F., Deschodt-Lanckman M., Vanherweghem J. L., Nortier J., Patterns of interstitial inflammation during the evolution of renal injury in experimental aristolochic acid nephropathy. Nephrol Dial Transplant, Vol. 23, pp. 2480-91 , 2008 Bibtex Bibtex : podzick08b Abstract : Background. Interstitial inflammation is a prominent feature associated with the severity of renal injury and progressive kidney failure. We utilized an animal model of aristolochic acid (AA)-induced nephropathy (AAN) to assess patterns of infiltration and inflammation during the evolution of tubulointerstitial damage and to relate them to the development of fibrosis. Methods. Male Wistar rats receiving sc daily AA or vehicle were sacrificed between Days 1 and 35. Infiltrating mononuclear cells were characterized by immunohistochemistry. The kidney infiltrating T lymphocytes were phenotyped by flow cytometry. Urinary levels of Th-1/ Th-2 cytokines, of monocyte chemoattractant protein-1 and of active transforming growth factor-beta (TGF-beta) were measured. Tissue expression of phosphorylated smad 2/3 protein was used to examine the TGF-beta signalling pathway. Results. In AA rats, monocytes/macrophages and T lymphocytes predominantly infiltrated areas of necrotic proximal tubular cells. The coexpressions of ED1 and/or Ki-67/MHCII by infiltrating cells reflected monocyte/macrophage proliferation and their activation, respectively. The accumulation of cytotoxic T lymphocytes was attested by severe signs of CD8+ cell tubulitis. The CD8/E-cadherin costaining confirmed intrarenal homing of CD8+CD103+ cells. Urinary levels of proinflammatory cytokines and of active TGF-beta significantly increased at Days 10 and 35. An early and persistent nuclear overexpression of phosphorylated smad 2/3 protein was detected in tubular and interstitial compartments. Conclusion. An early and massive interstitial inflammation characterized by activated monocytes/macrophages and cytotoxic CD8+CD103+ T lymphocytes is demonstrated for the first time during the progression of experimental AAN. The involvement in an interstitial fibrosis onset of active TGF-beta is highly suggested, at least via the psmad2/3 intracellular signalling pathway.Maris C, Rorive S, Sandras F, D'Haene N, Sadeghi N, Bieche I, Vidaud M, Decaestecker C, Salmon I, Tenascin-C expression relates to clinicopathological features in pilocytic and diffuse astrocytomas. Neuropathology and applied neurobiology, Vol. 34, 3, pp. 316-329, 2008 Bibtex Bibtex : maris08 Abstract : AIMS: Tenascin-C (TN-C) is an extracellular matrix brain glycoprotein for which conflicting in vitro and in vivo
results are reported in the literature dealing with its
involvement in astrocytoma aggressiveness, in particular
astrocytoma invasion. In view of these conflicting results
and the lack of data available on low-grade astrocytomas,
the present study focuses on pilocytic World Health
Organization (WHO) grade I, and diffuse WHO grade II
astrocytomas, that is, two histological entities associated
with very different invasive abilities. METHODS: Using
real-time reverse transcription polymerase chain reaction
and immunohistochemistry, we analysed the TN-C expression in
normal brain tissue as well as in a series of 54 pilocytic
and 53 grade II astrocytomas. CONCLUSIONS: Our data on
normal brain showed that while TN-C is largely expressed in
supratentorial white matter, it was largely absent in
infratentorial white matter. Paralleling these observations,
we showed that TN-C expression in low-grade astrocytomas
similarly varies according to tumour site. Cox regression
analysis evidenced that TN-C provided an independent
prognostic value which is enhanced in the case of grade II
astrocytomas for which positive TN-C expression is
associated with a higher risk of recurrence. We also
analysed TN-C expression specifically in vascular areas of
low-grade astrocytomas without demonstrating any prognostic
value for this additional feature. RESULTS: Similarly to
normal brain, low-grade astrocytomas exhibit variations in
TN-C expression with site, and this expression is associated
with an independent prognostic value in terms of recurrence.Saussez S., Lorfevre F., Lequeux T., Laurent G., Chantrain G., Vertongen F., Toubeau G., Decaestecker C., Kiss R., The determination of the levels of circulating galectin-1 and galectin-3 in HNSCC patients could be used to monitor tumor progression and/or responses to therapy. Oral Oncol, Vol. 44, pp. 86-93, 2008 Bibtex Bibtex : saussez07b Demetter P., Nagy N., Martin B., Mathieu A., Dumont P., Decaestecker C., Salmon I., The galectin family and digestive disease J Pathol, Vol. 215, 1, pp. 1-12, 2008 Bibtex Bibtex : demetter08 Mégalizzi V., Mathieu V., Mijatovic T., Gailly P., Debeir O., De Neve N., Van Damme M., Bontempi G., Haibe-Kains B., Decaestecker C., Kondo Y., Kiss R., Lefranc F., 4-IBP, a sigma1 receptor agonist, decreases the migration of human cancer cells, including glioblastoma cells, in vitro and sensitizes them in vitro and in vivo to cytotoxic insults of proapoptotic and proautophagic drugs. Neoplasia, Vol. 9, 5, pp. 358-69, May 2007 Bibtex Bibtex : megalizzi07 Decaestecker C., Debeir O., Van Ham P., Kiss R., Can anti-migratory drugs be screened in vitro? A review of 2D and 3D assays for the quantitative analysis of cell migration. Med Res Rev, Vol. 27, 2, pp. 149-76, March 2007 Bibtex Bibtex : decaestecker07r Saussez S., Decaestecker C., Lorfevre F., Cucu DR., Chevalier D., Wacreniez A., Kaltner H., André S., Toubeau G., Camby I., Gabius H. J., Kiss R., High level of galectin-1 expression is a negative prognostic predictor of recurrence in laryngeal squamous cell carcinomas. Int J Oncol, Vol. 30, pp. 1109-17, 2007 Bibtex Bibtex : saussez07 Mijatovic, T., Gailly, P., Mathieu, V., De Nève, N., Yeaton, P., Kiss, R., Decaestecker, C., Neurotensin is a versatile modulator of in vitro human pancreatic ductal adenocarcinoma cell (PDAC) migration Cell. Oncol., Vol. 29, pp. 315--326, 2007 Bibtex Bibtex : mijatovic07 Abstract : BACKGROUND: While the neurotensin (NT) roles in pancreatic cancer growth are well documented, its effects on pancreatic cancer cell migration have not been described. METHODS: The NT-induced effects on the migration process of human pancreatic ductal adenocarcinoma cells (PDACs) were characterized by means of various assays including computer-assisted video-microscopy, fluorescence microscopy, ELISA-based, small GTPase pull-down and phosphorylation assays. RESULTS: The NT-induced modifications on in vitro PDACs migration largely depended on the extra-cellular matrix environment and cell propensity to migrate collectively or individually. While NT significantly reduced the level of migration of collectively migrating PDACs on vitronectin, it significantly increased the level of individually migrating PDACs. These effects were mainly mediated through the sortilin/NTR3 receptor. Neurotensin both induced altered expression of alphaV and beta5 integrin subunits in PDACs cultured on vitronectin resulting in modified adhesion abilities, and caused modifications to the organization of the actin cytoskeleton through the NT-mediated activation of small Rho GTPases. While the NT effects on individually migrating PDACs were mediated at least through the EGFR/ERK signaling pathways, those on collectively migrating PDACs appeared highly dependent on the PI 3-kinase pathway. CONCLUSION: This study strongly suggests the involvement of neurotensin in the modulation of human PDAC migration. Sadeghi N., Salmon I., Decaestecker C., Levivier M., Metens T., Wikler D., Denolin V., Rorive S., Massager N., Baleriaux D., Goldman S., Stereotactic comparison between cerebral blood volume, methionine uptake, and histopathology in brain glioma. Am J Neuroradiol, Vol. 28, pp. 455-61, 2007 Bibtex Bibtex : sadeghi07 Servotte S., Camby I., Debeir O., Deroanne C., Lambert C. A., Lapière C. M, Kiss R., Nusgens B., Decaestecker C., The In Vitro Influences of Neurotensin on the Motility Characteristics of Human U373 Glioblastoma Cells. Neuropathology and Applied Neurobiology, Vol. 32, 6, pp. 575-84, Dec 2006 Bibtex Bibtex : servotte05 Note : accepted for publication 2005 Rorive S., Maris C., Debeir O., Sandras F., Vidaud M., Bieche I., Salmon I., Decaestecker C., Exploring the Distinctive Biological Characteristics of Pilocytic and Low-Grade Diffuse Astrocytomas Using Microarray Gene Expression Profiles. J Neuropathol Exp Neurol., Vol. 65, 8, pp. 794-807, Aug 2006 Bibtex Bibtex : rorive06 Saussez S., Cucu D. R., Decaestecker C., Chevalier D., Kaltner H., André S., Wacreniez A., Toubeau G., Camby I., Gabius H. J., Kiss R., Galectin 7 (p53-induced gene 1): a new prognostic predictor of recurrence and survival in stage IV hypopharyngeal cancer Ann Surg Oncol, Vol. 13, 7, pp. 999-1009, July 2006 Bibtex Bibtex : saussez06b C. Hayot, O. Debeir, P. Van Ham, M. Van Damme, R. Kiss, C. Decaestecker, Characterization of the activities of actin-affecting drugs on tumor cell migration. Toxicology and Applied Pharmacology, Vol. 211, 1, pp. 30-40, Feb 2006 Bibtex Bibtex : hayot06 Dubois F., Yourassowsky C., Monnom O., Legros J. C., Debeir O., Van Ham P., Kiss R., Decaestecker C., Digital Holographic Microscopy for the Three-dimensional Dynamic Analysis of In vitro Cancer Cell Migration. Journal of Biomedical Optics, Vol. 11, 5, pp. 054032-5p., 2006 Bibtex Bibtex : dubois06 A. Daelemans, T. Leloup, C. Decaestecker, A. De Mey, New digital method for quantitative assessment of nasal morphology Scan. J. Plast. Recons., Vol. 40, pp. 335-344, 2006 Bibtex Bibtex : daelemans06 Lefranc F., Decaestecker C., Brotchi J., Heizmann C. W., Dewitte O., Kiss R., Mijatovic T., Co-expression/co-location of S100 proteins (S100B, S100A1 and S100A2) and protein kinase C (PKC-beta, -eta and -zeta) in a rat model of cerebral basilar artery vasospasm Neuropathol Appl Neurobiol, Vol. 31, 6, pp. 649-660, Dec 2005 Bibtex Bibtex : lefranc05a Saal I., Nagy N., Lensch M., Lohr M., Manning J. C., Decaestecker C., Andre S., Kiss R., Salmon I., Gabius H. J., Human galectin-2: expression profiling by RT-PCR/immunohistochemistry and its introduction as a histochemical tool for ligand localization Histol Histopathol, Vol. 20, 4, pp. 1191-1208, Oct 2005 Bibtex Bibtex : saal05 D'Haene N., Maris C., Sandras F., Dehou M. F., Remmelink M., Decaestecker C., Salmon I., The differential expression of Galectin-1 and Galectin-3 in normal lymphoid tissue and non-Hodgkin's and Hodgkin's lymphomas Int J Immunopathol Pharmacol, Vol. 18, 3, pp. 431-443, July 2005 Bibtex Bibtex : d'haene05 O. Debeir, P. Van Ham, R. Kiss, C. Decaestecker, Tracking of Migrating Cells Under Phase-Contrast Video Microscopy With Combined Mean-Shift Processes IEEE Transaction on Medical Imaging, Vol. 24, 6, pp. 697-711, June 2005 Bibtex Bibtex : debeir05 Lefranc F., Mijatovic T., Decaestecker C., Kaltner H., André S., Brotchi J., Salmon I., Gabius H. J., Kiss R., Monitoring the expression profiles of integrins and adhesion/growth-regulatory galectins in adamantinomatous craniopharyngiomas: their ability to regulate tumor adhesiveness to surrounding tissue and their contribution to prognosis Neurosurgery, Vol. 56, 4, pp. 763-776, Apr 2005 Bibtex Bibtex : lefranc05b Van Quaquebeke E., Simon G., Andre A., Dewelle J., El Yazidi M., Bruyneel F., Tuti J., Nacoulma O., Guissou P., Decaestecker C., Braekman J. C., Kiss R., Darro F., Identification of a novel cardenolide (2''-oxovoruscharin) from Calotropis procera and the hemisynthesis of novel derivatives displaying potent in vitro antitumor activities and high in vivo tolerance: structure-activity relationship analyses J Med Chem, Vol. 48, 3, pp. 849-856, Feb 2005 Bibtex Bibtex : van05 Remmelink M., Mijatovic T., Gustin A., Mathieu A., Rombaut K., Kiss R., Salmon I., Decaestecker C., Identification by means of cDNA microarray analyses of gene expression modifications in squamous non-small cell lung cancers as compared to normal bronchial epithelial tissue Int J Oncol, Vol. 26, 1, pp. 247-258, Jan 2005 Bibtex Bibtex : remmelink05 Darro F., Decaestecker C., Gaussin J. F., Mortier S., Van Ginckel R., Kiss R., Are syngeneic mouse tumor models still valuable experimental models in the field of anti-cancer drug discovery? Int J Oncol, Vol. 27, 3, pp. 607-616, 2005 Bibtex Bibtex : darro05 Camby I., Decaestecker C., Lefranc F., Kaltner H., Gabius H. J., Kiss R., Galectin-1 knocking down in human U87 glioblastoma cells alters their gene expression pattern Biochem Biophys Res Commun, Vol. 335, 1, pp. 27-35, 2005 Bibtex Bibtex : camby05 Mathieu A., Saal I., Vuckovic A., Ransy V., Vereerstraten P., Kaltner H., Gabius H.J., Kiss R., Decaestecker C., Salmon I., Remmelink M., Nuclear galectin-3 expression is an independent predictive factor of recurrence for adenocarcinoma and squamous cell carcinoma of the lung Mod Pathol, Vol. 18, 9, pp. 1264-1271, 2005 Bibtex Bibtex : mathieu05 F. Lefranc, T. Mijatovic, V. Mathieu, S. Rorive, C. Decaestecker, O. Debeir, J. Brotchi, P. Van Ham, I. Salmon, R. Kiss, Characterization of gastrin-induced proangiogenic effects in vivo in orthotopic u373 experimental human glioblastomas, and in vitro in human umbilical vein endothelial cells (huvec) Clin Cancer Res, Vol. 10, 24, pp. 8250-65, Dec 2004 Bibtex Bibtex : lefranc04 Hancq S., Salmon I., Brotchi J., Gabius H. J., Heizmann C. W., Kiss R., Decaestecker C., Detection of S100B, S100A6 and galectin-3 ligands in meningiomas as markers of aggressiveness Int J Oncol, Vol. 25, 5, pp. 1233-1240, Nov 2004 Bibtex Bibtex : hancq04b Legendre H., Decaestecker C., Gbenou M. Goris, Nagy N., Hendlisz A., Andre S., Pector J. C., Kiss R., Gabius H. J., Prognostic stratification of Dukes B colon cancer by a neoglycoprotein Int J Oncol, Vol. 25, 2, pp. 269-276, Aug 2004 Bibtex Bibtex : legendre04 O. Debeir, I. Camby, R. Kiss, P. Van Ham, C. Decaestecker, A Model-Based Approach For Automated In Vitro Cell Tracking And Chemotaxis Analyses Cytometry, Vol. 60A, 1, pp. 29-40, July 2004 Bibtex Bibtex : debeir04 Gaigneaux A., Decaestecker C., Camby I., Mijatovic T., Kiss R., Ruysschaert J. M., Goormaghtigh E., The infrared spectrum of human glioma cells is related to their in vitro and in vivo behavior Exp Cell Res, Vol. 297, 1, pp. 294-301, July 2004 Bibtex Bibtex : gaigneaux04 Hancq S., Salmon I., Brotchi J., De Witte O., Gabius H. J., Heizmann C. W., Kiss R., Decaestecker C., S100A5: a marker of recurrence in WHO grade I meningiomas Neuropathol Appl Neurobiol, Vol. 30, 2, pp. 178-187, Apr 2004 Bibtex Bibtex : hancq04a Hittelet A., Camby I., Nagy N., Legendre H., Bronckart Y., Decaestecker C., Kaltner H., Nifant'ev N. E., Bovin N. V., Pector J. C., Salmon I., Gabius H. J., Kiss R., Yeaton P., Binding sites for Lewis antigens are expressed by human colon cancer cells and negatively affect their migration Lab Invest, Vol. 83, 6, pp. 777-787, June 2003 Bibtex Bibtex : hittelet03 Delbrouck C., Gabius H. J., Kaltner H., Decaestecker C., Kiss R., Hassid S., Expression patterns of galectin-1 and galectin-3 in nasal polyps and middle and inferior turbinates in relation to growth regulation and immunosuppression Arch Otolaryngol Head Neck Surg, Vol. 129, 6, pp. 665-669, June 2003 Bibtex Bibtex : delbrouck03 Legendre H., Decaestecker C., Nagy N., Hendlisz A., Schuring M. P., Salmon I., Gabius H. J., Pector J. C., Kiss R., Prognostic values of galectin-3 and the macrophage migration inhibitory factor (MIF) in human colorectal cancers Mod Pathol, Vol. 16, 5, pp. 491-504, May 2003 Bibtex Bibtex : legendre03 Pelc P., Vanmuylder N., Lefranc F., Heizmann C. W., Hassid S., Salmon I., Kiss R., Louryan S., Decaestecker C., Differential expression of S100 calcium-binding proteins in epidermoid cysts, branchial cysts, craniopharyngiomas and cholesteatomas Histopathology, Vol. 42, 4, pp. 387-394, Apr 2003 Bibtex Bibtex : pelc03 Nagy N., Legendre H., Engels O., Andre S., Kaltner H., Wasano K., Zick Y., Pector J. C., Decaestecker C., Gabius H. J., Salmon I., Kiss R., Refined prognostic evaluation in colon carcinoma using immunohistochemical galectin fingerprinting Cancer, Vol. 97, 8, pp. 1849-1858, Apr 2003 Bibtex Bibtex : nagy03 Rorive S, Eddafali B, Fernandez S, Decaestecker C, Andre S, Kaltner H, Kuwabara I, Liu F-T, Gabius H-J, Kiss R, Salmon I, Changes in galectin-7 and cytokeratin-19 expression during the progression of malignancy in thyroid tumors: diagnostic and biological implications Mod Pathol, Vol. 15, 12, pp. 1294--1301, Dec 2002 Bibtex Bibtex : rorive02 Belot N, Pochet R, Heizmann C-W, Kiss R, Decaestecker C, Extracellular S100A4 stimulates the migration rate of astrocytic tumor cells by modifying the organization of their actin cytoskeleton Biochim Biophys Acta, Vol. 1600, 1-2, pp. 74--83, Nov 2002 Bibtex Bibtex : belot02 Lefranc F, Golzarian J, Chevalier C, Dewitte O, Pochet R, Heizman C, Decaestecker C, Brotchi J, Salmon I, Kiss R, Expression of members of the calcium-binding S-100 protein family in a rat model of cerebral basilar artery vasospasm J Neurosurg, Vol. 97, 2, pp. 408--415, Aug 2002 Bibtex Bibtex : lefranc02 Hayot C, Farinelle S, De Decker R, Decaestecker C, Darro F, Kiss R, Van Damme M, In vitro pharmacological characterizations of the anti-angiogenic and anti-tumor cell migration properties mediated by microtubule-affecting drugs, with special emphasis on the organization of the actin cytoskeleton Int J Oncol, Vol. 21, 2, pp. 417--425, Aug 2002 Bibtex Bibtex : hayot02 Pelc K, Vincent S, Ruchoux M-M, Kiss R, Pochet R, Sariban E, Decaestecker C, Heizmann C W, Calbindin-d(28k): a marker of recurrence for medulloblastomas Cancer, Vol. 95, 2, pp. 410--419, July 2002 Bibtex Bibtex : pelc02 Joseph B, Darro F, Behard A, Lesur B, Collignon F, Decaestecker C, Frydman A, Guillaumet G, Kiss R, 3-Aryl-2-quinolone derivatives: synthesis and characterization of in vitro and in vivo antitumor effects with emphasis on a new therapeutical target connected with cell migration J Med Chem, Vol. 45, 12, pp. 2543--2555, June 2002 Bibtex Bibtex : joseph02 Nemec E, Van de Putte S, Van Pachterbeke C, Vokaer R, Budel V, Deprez C, Kiss R, Decaestecker C, Ploidy and chromatin pattern analysis as an aid for cervical smear diagnosis Histol Histopathol, Vol. 17, 2, pp. 403--409, Apr 2002 Bibtex Bibtex : nemec02 Delbrouck C, Doyen I, Belot N, Decaestecker C, Ghanooni R, de Lavareille A, Kaltner H, Choufani G, Danguy A, Vandenhoven G, Gabius H-J, Hassid S, Kiss R, Galectin-1 is overexpressed in nasal polyps under budesonide and inhibits eosinophil migration Lab Invest, Vol. 82, 2, pp. 147--158, Feb 2002 Bibtex Bibtex : delbrouck02 Saerens M, Latinne P, Decaestecker C, Adjusting the outputs of a classifier to new a priori probabilities: a simple procedure Neural Comput, Vol. 14, 1, pp. 21--41, Jan 2002 Bibtex Bibtex : saerens02 Nagy N, Hoyaux D, Gielen I, Schafer B W, Pochet R, Heizmann C W, Kiss R, Salmon I, Decaestecker C, The Ca2+-binding S100A2 protein is differentially expressed in epithelial tissue of glandular or squamous origin Histol Histopathol, Vol. 17, 1, pp. 123--130, Jan 2002 Bibtex Bibtex : nagy02 Latinne P., Debeir O., Decaestecker C., Combining Different Methods and Numbers of Weak Decision Trees Pattern Analysis and Applications (special issue on Fusion of Multiple Classifiers), Vol. 5, 2, pp. 201-209, 2002 Bibtex Bibtex : latinne02 Hassid S, Afrapoli A H, Decaestecker C, Choufani G, UPPP for snoring: long-term results and patient satisfaction Acta Otorhinolaryngol Belg, Vol. 56, 2, pp. 157--162, 2002 Bibtex Bibtex : hassid02 Belot N, Rorive S, Doyen I, Lefranc F, Bruyneel E, Dedecker R, Micik S, Brotchi J, Decaestecker C, Salmon,I, Kiss R, Camby I, Molecular characterization of cell substratum attachments in human glial tumors relates to prognostic features Glia, Vol. 36, 3, pp. 375--390, Dec 2001 Bibtex Bibtex : belot01 Kucharczak J, Pannequin J, Camby I, Decaestecker C, Kiss R, Martinez J, Gastrin induces over-expression of genes involved in human U373 glioblastoma cell migration Oncogene, Vol. 20, 48, pp. 7021--7028, Oct 2001 Bibtex Bibtex : kucharczak01 Bronckart Y, Decaestecker C, Nagy N, Harper L, Schafer B W, Salmon I, Pochet R, Kiss R, Heizman C W, Development and progression of malignancy in human colon tissues are correlated with expression of specific Ca(2+)-binding S100 proteins Histol Histopathol, Vol. 16, 3, pp. 707--712, July 2001 Bibtex Bibtex : bronckart01 Danguy A, Rorive S, Decaestecker C, Bronckart Y, Kaltner H, Hadari Y R, Goren R, Zich Y, Petein M, Salmon I, Gabius H J, Kiss R, Immunohistochemical profile of galectin-8 expression in benign and malignant tumors of epithelial, mesenchymatous and adipous origins, and of the nervous system Histol Histopathol, Vol. 16, 3, pp. 861--868, July 2001 Bibtex Bibtex : danguy01 Berthe J V, Remmelink M, Werry J, Salmon I, Kiss R, Decaestecker C, The contribution of image cytometry to the characterization of clinical subgroups of lipomas Int J Oncol, Vol. 18, 6, pp. 1315--1321, June 2001 Bibtex Bibtex : berthe01 Sheikholeslam-Zadeh R, Decaestecker C, Delbrouck C, Danguy A, Salmon I, Zick Y, Kaltner H, Hassid S, Gabius H J, Kiss R, Choufani G, The levels of expression of galectin-3, but not of galectin-1 and galectin-8, correlate with apoptosis in human cholesteatomas Laryngoscope, Vol. 111, 6, pp. 1042--1047, June 2001 Bibtex Bibtex : sheikholeslam-zadeh01 Simon P, Decaestecker C, Choufani G, Delbrouck C, Danguy A, Salmon I, Zick Y, Kaltner H, Hassid S, Gabius H J, Kiss R, Darro F, The levels of retinoid RARbeta receptors correlate with galectin-1, -3 and -8 expression in human cholesteatomas Hear Res, Vol. 156, 1-2, pp. 1--9, June 2001 Bibtex Bibtex : simon01 Nagy N, Brenner C, Markadieu N, Chaboteaux C, Camby I, Schafer B W, Pochet R, Heizmann C W, Salmon I, Kiss R, Decaestecker C, S100A2, a putative tumor suppressor gene, regulates in vitro squamous cell carcinoma migration Lab Invest, Vol. 81, 4, pp. 599--612, Apr 2001 Bibtex Bibtex : nagy01 Rorive S, Belot N, Decaestecker C, Lefranc F, Gordower L, Micik S, Maurage C A, Kaltner H, Ruchoux M M, Danguy A, Gabius H J, Salmon I, Kiss R, Camby I, Galectin-1 is highly expressed in human gliomas with relevance for modulation of invasion of tumor astrocytes into the brain parenchyma Glia, Vol. 33, 3, pp. 241--255, March 2001 Bibtex Bibtex : rorive01 Camby I, Decaestecker C, Gordower L, Dedecker R, Kacem Y, Lemmers A, Siebert H C, Bovin N V, Wesseling P, Danguy A, Salmon I, Gabius H J, Kiss R, Distinct differences in binding capacity to saccharide epitopes in supratentorial pilocytic astrocytomas, astrocytomas, anaplastic astrocytomas, and glioblastomas J Neuropathol Exp Neurol, Vol. 60, 1, pp. 75--84, Jan 2001 Bibtex Bibtex : camby01 Pilette C, Godding V, Kiss R, Delos M, Verbeken E, Decaestecker C, De Paepe K, Vaerman J P, Decramer M, Sibille Y, Reduced epithelial expression of secretory component in small airways correlates with airflow obstruction in chronic obstructive pulmonary disease Am J Respir Crit Care Med, Vol. 163, 1, pp. 185--194, Jan 2001 Bibtex Bibtex : pilette01 Choufani G, Ghanooni R, Decaestecker C, Delbrouck K, Simon P, Schuring M P, Zick Y, Hassid S, Gabius H J, Kiss R, Detection of macrophage migration inhibitory factor (MIF) in human cholesteatomas and functional implications of correlations to recurrence status and to expression of matrix metalloproteinases-3/9, retinoic acid receptor-beta, and anti-apoptotic galectin-3 Laryngoscope, Vol. 111, 9, pp. 1656--1662, 2001 Bibtex Bibtex : choufani01 Delfourne E, Darro F, Bontemps-Subielos N, Decaestecker C, Bastide J, Frydman A, Kiss R, Synthesis and characterization of the antitumor activities of analogues of meridine, a marine pyridoacridine alkaloid J Med Chem, Vol. 44, 20, pp. 3275--3282, 2001 Bibtex Bibtex : delfourne01 van Velthoven R, Petein M, Oosterlinck W, De Wilde T, Mattelaer J, Hardeman M, Kiss R, Decaestecker C, Identification by quantitative chromatin pattern analysis of patients at risk for recurrence of superficial transitional bladder carcinoma J Urol, Vol. 164, 6, pp. 2134--2137, Dec 2000 Bibtex Bibtex : van00 Steinfeld S, Penaloza A, Decaestecker C, Rommes S, Andre S, Schuring M P, Danguy A, Appelboom T, Kiss R, Gabius H J, Labeled neoglycoproteins and human lectins as diagnostic and potential functional markers in salivary glands of patients with Sjogren's syndrome J Rheumatol, Vol. 27, 8, pp. 1910--1916, Aug 2000 Bibtex Bibtex : steinfeld00a Hassid S, Choufani G, Decaestecker C, Delbrouck C, Dawance S, Pelc P, Nagy N, Kaltner H, Salmon I, Danguy A, Gabius H J, Kiss R, Glycohistochemical characteristics of nasal polyps from patients with and without cystic fibrosis Arch Otolaryngol Head Neck Surg, Vol. 126, 6, pp. 769--776, June 2000 Bibtex Bibtex : hassid00 Hoyaux D, Decaestecker C, Heizmann C W, Vogl T, Schafer B W, Salmon I, Kiss R, Pochet R, S100 proteins in Corpora amylacea from normal human brain Brain Res, Vol. 867, 1-2, pp. 280--288, June 2000 Bibtex Bibtex : hoyaux00 Darro F, Schwarz G Jr, Petein M, Schwarz S, Chaboteaux C, Nogaret J M, Decaestecker C, Salmon I, Kiss R, Characterization of steroid hormone sensitivity in human breast cancers maintained ex vivo under organotypical culture conditions J Cancer Res Clin Oncol, Vol. 126, 5, pp. 257--262, May 2000 Bibtex Bibtex : darro00 Nagy N, Decaestecker C, Dong X, Kaltner H, Schuring M P, Rocmans P, Danguy A, Gabius H J, Kiss R, Salmon I, Characterization of ligands for galectins, natural galactoside-binding immunoglobulin G subfractions and sarcolectin and also of the expression of calcyclin in thyroid lesions Histol Histopathol, Vol. 15, 2, pp. 503--513, Apr 2000 Bibtex Bibtex : nagy00 Hittelet A B, Yeaton P, Decaestecker C, Remmelink M, Nagy N, Cremer M, Salmon I, Kiss R, Bourgeois N, Discrimination between dysplastic and malignant epithelium of the ampulla of vater based on quantitative image cytometric data Anal Quant Cytol Histol, Vol. 22, 2, pp. 98--106, Apr 2000 Bibtex Bibtex : hittelet00 Philippart P, Harper L, Chaboteaux C, Decaestecker C, Bronckart Y, Gordover L, Lesueur-Ginot L, Malonne H, Lavergne O, Bigg D C, da Costa P M, Kiss R, Homocamptothecin, an E-ring-modified camptothecin, exerts more potent antiproliferative activity than other topoisomerase I inhibitors in human colon cancers obtained from surgery and maintained in vitro under histotypical culture conditions Clin Cancer Res, Vol. 6, 4, pp. 1557--1562, Apr 2000 Bibtex Bibtex : philippart00 Steinfeld S, Rommes S, Francois C, Decaestecker C, Maho A, Appelboom T, Heizmann C W, Kiss R, Pochet R, Big prolactin 60 kDa is overexpressed in salivary glandular epithelial cells from patients with Sjogren's syndrome Lab Invest, Vol. 80, 2, pp. 239--247, Feb 2000 Bibtex Bibtex : steinfeld00b Camby I, Lefranc F, Titeca G, Neuci S, Fastrez M, Dedecken L, Schafer B W, Brotchi J, Heizmann C W, Pochet R, Salmon I, Kiss R, Decaestecker C, Differential expression of S100 calcium-binding proteins characterizes distinct clinical entities in both WHO grade II and III astrocytic tumours Neuropathol Appl Neurobiol, Vol. 26, 1, pp. 76--90, Feb 2000 Bibtex Bibtex : camby00 Francois C, Moreno C, Teitelbaum J, Bigras G, Salmon I, Danguy A, Brugal G, van Velthoven R, Kiss R, Decaestecker C, Improving accuracy in the grading of renal cell carcinoma by combining the quantitative description of chromatin pattern with the quantitative determination of cell kinetic parameters Cytometry, Vol. 42, 1, pp. 18--26, Feb 2000 Bibtex Bibtex : francois00 Farinelle S, Malonne H, Chaboteaux C, Decaestecker C, Dedecker R, Gras T, Darro F, Fontaine J, Atassi G, Kiss R, Characterization of TNP-470-induced modifications to cell functions in HUVEC and cancer cells J Pharmacol Toxicol Methods, Vol. 43, 1, pp. 15--24, Jan 2000 Bibtex Bibtex : farinelle00 Remmelinck M, Lopes M B, Nagy N, Rorive S, Rombaut K, Decaestecker C, Kiss R, Salmon I, How could static telepathology improve diagnosis in neuropathology? Anal Cell Pathol, Vol. 21, 3-4, pp. 177--182, 2000 Bibtex Bibtex : remmelinck00 Malonne H, Farinelle S, Decaestecker C, Gordower L, Fontaine J, Chaminade F, Saucier J M, Atassi G, Kiss R, In vitro and in vivo pharmacological characterizations of the antitumor properties of two new olivacine derivatives, S16020-2 and S30972-1 Clin Cancer Res, Vol. 6, 9, pp. 3774--3782, 2000 Bibtex Bibtex : malonne00 Choufani G, Mahillon V, Decaestecker C, Lequeux T, Danguy A, Salmon I, Gabius H J, Hassid S, Kiss R, Determination of the levels of expression of sarcolectin and calcyclin and of the percentages of apoptotic but not proliferating cells to enable distinction between recurrent and nonrecurrent cholesteatomas Laryngoscope, Vol. 109, 11, pp. 1825--1831, Nov 1999 Bibtex Bibtex : choufani99 Penaloza A, Decaestecker C, Ribai P, Nagy N, Salmon I, Appelboom T, Danguy A, Kiss R, Steinfeld S, Sialic acid residues in the labial salivary glands from Sjogren's syndrome patients Clin Exp Rheumatol, Vol. 17, 6, pp. 713--717, Nov 1999 Bibtex Bibtex : penaloza99 Bronckart Y, Nagy N, Decaestecker C, Bouckaert Y, Remmelink M, Gielen I, Hittelet A, Darro F, Pector J C, Yeaton P, Danguy A, Kiss R, Salmon I, Grading dysplasia in colorectal adenomas by means of the quantitative binding pattern determination of Arachis hypogaea, Dolichos biflorus, Amaranthus caudatus, Maackia amurensis, and Sambucus nigra agglutinins Hum Pathol, Vol. 30, 10, pp. 1178--1191, Oct 1999 Bibtex Bibtex : bronckart99 Francois, C, van Velthoven, R, De Lathouwer, O, Moreno, C, Peltier, A, Kaltner, H, Salmon, I, Gabius, H J, Danguy, A, Decaestecker, C, Kiss, R, Galectin-1 and galectin-3 binding pattern expression in renal cell carcinomas Am J Clin Pathol, Vol. 112, 2, pp. 194--203, Aug 1999 Bibtex Bibtex : francois99a Gordower, L, Decaestecker, C, Kacem, Y, Lemmers, A, Gusman, J, Burchert, M, Danguy, A, Gabius, H, Salmon, I, Kiss, R, Camby, I, Galectin-3 and galectin-3-binding site expression in human adult astrocytic tumours and related angiogenesis Neuropathol Appl Neurobiol, Vol. 25, 4, pp. 319--330, Aug 1999 Bibtex Bibtex : gordower99 Schwarz, G Jr, Remmelink, M, Decaestecker, C, Gielen, I, Budel, V, Burchert, M, Darro, F, Danguy, A, Gabius, H J, Salmon, I, Kiss, R, Galectin fingerprinting in tumor diagnosis. Differential expression of galectin-3 and galectin-3 binding sites, but not galectin-1, in benign vs malignant uterine smooth muscle tumors Am J Clin Pathol, Vol. 111, 5, pp. 623--631, May 1999 Bibtex Bibtex : schwarz99 De Hauwer, C., Darro, F., Camby, I., Kiss, R., Van Ham, P., Decaestecker, C., In vitro motility evaluation of aggregated cancer cells by means of automatic image processing Cytometry, Vol. 36, pp. 1--10, May 1999 Bibtex Bibtex : de hauwer99 Steinfeld S, Penaloza A, Ribai P, Decaestecker C, Danguy A, Gabius H J, Salmon I, Appelboom T, Kiss R, D-mannose and N-acetylglucosamine moieties and their respective binding sites in salivary glands of Sjogren's syndrome J Rheumatol, Vol. 26, 4, pp. 833--841, Apr 1999 Bibtex Bibtex : steinfeld99 Francois, C, Decaestecker, C, De Lathouwer, O, Moreno, C, Peltier, A, Roumeguere, T, Danguy, A, Pasteels, J L, Wespes, E, Salmon, I, van Velthoven, R, Kiss, R, Improving the prognostic value of histopathological grading and clinical staging in renal cell carcinomas by means of computer-assisted microscopy J Pathol, Vol. 187, 3, pp. 313--320, Feb 1999 Bibtex Bibtex : francois99b Camby, I, Nagy, N, Lopes, M B, Schafer, B W, Maurage, C A, Ruchoux, M M, Murmann, P, Pochet, R, Heizmann, C W, Brotchi, J, Salmon, I, Kiss, R, Decaestecker, C, Supratentorial pilocytic astrocytomas, astrocytomas, anaplastic astrocytomas and glioblastomas are characterized by a differential expression of S100 proteins Brain Pathol, Vol. 9, 1, pp. 1--19, Jan 1999 Bibtex Bibtex : camby99 Nagy, N, Decaestecker, C, Kiss, R, Rypens, F, Van Gansbeke, D, Mockel, J, Rocmans, P, Salmon, I, A pilot study for identifying at risk thyroid lesions by means of a decision tree run on clinicocytological variables Int J Mol Med, Vol. 4, 3, pp. 299--308, 1999 Bibtex Bibtex : nagy99a O. Debeir, C. Decaestecker, J. L. Pasteels, I. Salmon, R. Kiss, P. Van Ham, Computer-assisted analysis of epiluminescence microscopy images of pigmented skin lesions Cytometry, Vol. 37, 4, pp. 255-66, 1999 Bibtex Bibtex : debeir99 Remmelink, M, Darro, F, Decaestecker, C, De Decker, R, Bovin, N V, Gebhart, M, Kaltner, H, Gabius, H J, Kiss, R, Salmon, I, Danguy, A, In vitro influence of lectins and neoglycoconjugates on the growth of three human sarcoma cell lines J Cancer Res Clin Oncol, Vol. 125, 5, pp. 275--285, 1999 Bibtex Bibtex : remmelink99 Nagy, N, Camby, I, Decaestecker, C, Chaboteaux, C, Gras, T, Darro, F, Rocmans, P, Kiss, R, Salmon, I, The influence of L-triiodothyronine, L-thyroxine, estradiol-17beta, the luteinizing-hormone-releasing hormone, the epidermal growth factor and gastrin on cell proliferation in organ cultures of 35 benign and 13 malignant human thyroid tumors J Cancer Res Clin Oncol, Vol. 125, 6, pp. 361--368, 1999 Bibtex Bibtex : nagy99b Wespes, E, Raviv, G, Vanegas, J P, Decaestecker, C, Petein, M, Danguy, A, Schulman, C C, Kiss, R, Corporeal veno-occlusive dysfunction: a distal arterial pathology? J Urol, Vol. 160, 6 Pt 1, pp. 2054--2057, Dec 1998 Bibtex Bibtex : wespes98 Yeaton P, Frierson H F Jr, Hittelet A, Duckworth C, Deprez C, Bourgeois N, Salmon I, Jones R S, Kiss R, Decaestecker C, Use of image cytometry to classify biliary and ampullary adenocarcinomas Anal Quant Cytol Histol, Vol. 20, 6, pp. 509--516, Dec 1998 Bibtex Bibtex : yeaton98a De Hauwer, C, Camby, I, Darro, F, Migeotte, I, Decaestecker, C, Verbeek, C, Danguy, A, Pasteels, J L, Brotchi, J, Salmon, I, Van Ham, P, Kiss, R, Gastrin inhibits motility, decreases cell death levels and increases proliferation in human glioblastoma cell lines J Neurobiol, Vol. 37, 3, pp. 373--382, Nov 1998 Bibtex Bibtex : de98 Farinelle S, Dehauwer C, Darro F, Decaestecker C, Fontaine J, Pasteels JL, Van Ham P, Atassi G, Kiss R, Setting up of an original computer-assisted methodology to characterize in vitro drug-induced anti-angiogenic effects Int J Mol Med, Vol. 2, 5, pp. 545--553, Nov 1998 Bibtex Bibtex : farinelle98 Note : JOURNAL ARTICLE Decaestecker, C, Camby, I, Gordower, L, Dewitte, O, Cras, P, Martin, J J, Pasteels, J L, Van Ham, P, Brotchi, J, Kiss, R, Salmon, I, Characterization of astroglial versus oligodendroglial phenotypes in glioblastomas by means of quantitative morphonuclear variables generated by computer-assisted microscopy J Neuropathol Exp Neurol, Vol. 57, 8, pp. 791--802, Aug 1998 Bibtex Bibtex : decaestecker98a Yeaton, P, Sears, R J, Ledent, T, Salmon, I, Kiss, R, Decaestecker, C, Discrimination between chronic pancreatitis and pancreatic adenocarcinoma using artificial intelligence-related algorithms based on image cytometry-generated variables Cytometry, Vol. 32, 4, pp. 309--316, Aug 1998 Bibtex Bibtex : yeaton98b Francois, C, Decaestecker, C, Salmon, I, Petein, M, Remmelink, M, Janssen, T, Peltier, A, Wespes, E, Schulman, C, van Velthoven, R, Kiss, R, Prognostic value of stem cell line identification for renal cell carcinomas Anal Quant Cytol Histol, Vol. 20, 3, pp. 207--214, June 1998 Bibtex Bibtex : francois98a Kiss, R, Decaestecker, C, Camby, I, Darro, F, Salmon, I, Danguy, A, Pasteels, J L, Yeaton, P, Direct relationship between hormone sensitivity level and growth pattern. Evidence in 18 gastrointestinal neoplastic cell lines Anal Quant Cytol Histol, Vol. 20, 2, pp. 133--143, Apr 1998 Bibtex Bibtex : kiss98 Sears R J, Duckworth C W, Decaestecker C, Bourgeois N, Ledent T, Deviere J, Salmon I, Kiss R, Yeaton P, Image cytometry as a discriminatory tool for cytologic specimens obtained by endoscopic retrograde cholangiopancreatography Cancer, Vol. 84, 2, pp. 119--126, Apr 1998 Bibtex Bibtex : sears98 Decaestecker, C, Camby, I, Nagy, N, Brotchi, J, Kiss, R, Salmon, I, Improving morphology-based malignancy grading schemes in astrocytic tumors by means of computer-assisted techniques Brain Pathol, Vol. 8, 1, pp. 29--38, Jan 1998 Bibtex Bibtex : decaestecker98b Saussez, S, Marchant, H, Nagy, N, Decaestecker, C, Hassid, S, Jortay, A, Schuring, M P, Gabius, H J, Danguy, A, Salmon, I, Kiss, R, Quantitative glycohistochemistry defines new prognostic markers for cancers of the oral cavity Cancer, Vol. 82, 2, pp. 252--260, Jan 1998 Bibtex Bibtex : saussez98 Danguy, A, Decaestecker, C, Genten, F, Salmon, I, Kiss, R, Applications of lectins and neoglycoconjugates in histology and pathology Acta Anat (Basel), Vol. 161, 1-4, pp. 206--218, 1998 Bibtex Bibtex : danguy98 Gordower, L, Decaestecker, C, Lopes, M B, Camby, I, Nagy, N, Francois, C, Cras, P, Martin, J J, Brotchi, J, Kiss, R, Salmon, I, Determination of growth fraction and cell density to evaluate the potential growth of human oligodendroglial and astrocytic tumours J Cancer Res Clin Oncol, Vol. 124, 8, pp. 427--434, 1998 Bibtex Bibtex : gordower98 Darro, F, Cahen, P, Vianna, A, Decaestecker, C, Nogaret, J M, Leblond, B, Chaboteaux, C, Ramos, C, Petein, M, Budel, V, Schoofs, A, Pourrias, B, Kiss, R, Growth inhibition of human in vitro and mouse in vitro and in vivo mammary tumor models by retinoids in comparison with tamoxifen and the RU-486 anti-progestagen Breast Cancer Res Treat, Vol. 51, 1, pp. 39--55, 1998 Bibtex Bibtex : darro98 Francois, C, Remmelink, M, Petein, M, van Velthoven, R, Danguy, A, Wespes, E, Salmon, I, Kiss, R, Decaestecker, C, The chromatin pattern of cell nuclei is of prognostic value for renal cell carcinomas Anal Cell Pathol, Vol. 16, 3, pp. 161--175, 1998 Bibtex Bibtex : francois98b Remmelink, M, Decaestecker, C, Darro, F, Goldschmidt, D, Gebhart, M, Pasteels, J L, Kiss, R, Salmon, I, The in vitro influence of eight hormones and growth factors on the proliferation of eight sarcoma cell lines J Cancer Res Clin Oncol, Vol. 124, 3-4, pp. 155--164, 1998 Bibtex Bibtex : remmelink98 Hassid, S, Decaestecker, C, Hermans, C, Salmon, I, Pasteels, J L, Danguy, A, Kiss, R, Algorithm analysis of lectin glycohistochemistry and Feulgen cytometry for a new classification of nasal polyposis Ann Otol Rhinol Laryngol, Vol. 106, 12, pp. 1043--1051, Dec 1997 Bibtex Bibtex : hassid97a Hassid, S, Degaute, M P, Dawance, S, Rombaut, K, Nagy, N, Choufani, G, Decaestecker, C, Danguy, A, Salmon, I, Kiss, R, Determination of proliferative activity in nasal polyps J Clin Pathol, Vol. 50, 11, pp. 923--928, Nov 1997 Bibtex Bibtex : hassid97b Francois, C, Decaestecker, C, Petein, M, Van Ham, P, Peltier, A, Pasteels, J L, Danguy, A, Salmon, I, van Velthoven, R, Kiss, R, Classification strategies for the grading of renal cell carcinomas, based on nuclear morphometry and densitometry J Pathol, Vol. 183, 2, pp. 141--150, Oct 1997 Bibtex Bibtex : francois97 Lorea P, Decaestecker C, Goldschmidt D, Renard N, Andre J, Lipski D, Van den Heule B, Salmon I, Darro F, Kiss R, Correlation between gender and cytomorphonuclear characteristics in human melanomas and in vitro evidence of sex steroid-induced modifications in the morphonuclear characteristics of three human melanoma cell lines Melanoma Res, Vol. 7, 5, pp. 382--392, Oct 1997 Bibtex Bibtex : lorea97 Goldschmidt, D, Gordower, L, Berthe, J V, Remmelink, M, Decaestecker, C, Petein, M, Salmon, I, Kiss, R, Danguy, A, Contribution of quantitative lectin histochemistry to characterizing well-differentiated, dedifferentiated and poorly differentiated liposarcomas Anal Quant Cytol Histol, Vol. 19, 3, pp. 215--226, June 1997 Bibtex Bibtex : goldschmidt97 Decaestecker, C, Camby, I, Remmelink, M, Nagy, N, Petein, M, Pasteels, J L, Van Ham, P, Salmon, I, Kiss, R, Decision tree induction: a useful tool for assisted diagnosis and prognosis in tumor pathology Lab Invest, Vol. 76, 6, pp. 799--808, June 1997 Bibtex Bibtex : decaestecker97c Decaestecker, C, Lopes, B S, Gordower, L, Camby, I, Cras, P, Martin, J J, Kiss, R, VandenBerg, S R, Salmon, I, Quantitative chromatin pattern description in Feulgen-stained nuclei as a diagnostic tool to characterize the oligodendroglial and astroglial components in mixed oligo-astrocytomas J Neuropathol Exp Neurol, Vol. 56, 4, pp. 391--402, Apr 1997 Bibtex Bibtex : decaestecker97b De Hauwer, C, Camby, I, Darro, F, Decaestecker, C, Gras, T, Salmon, I, Kiss, R, Van Ham, P, Dynamic characterization of glioblastoma cell motility Biochem Biophys Res Commun, Vol. 232, 2, pp. 267--272, March 1997 Bibtex Bibtex : de97 Decaestecker, C, Salmon, I, Dewitte, O, Camby, I, Van Ham, P, Pasteels, J L, Brotchi, J, Kiss, R, Nearest-neighbor classification for identification of aggressive versus nonaggressive low-grade astrocytic tumors by means of image cytometry-generated variables J Neurosurg, Vol. 86, 3, pp. 532--537, March 1997 Bibtex Bibtex : decaestecker97a Kiss, R, Dewitte, O, Decaestecker, C, Camby, I, Gordower, L, Delbecque, K, Pasteels, J L, Brotchi, J, Salmon, I, The combined determination of proliferative activity and cell density in the prognosis of adult patients with supratentorial high-grade astrocytic tumors Am J Clin Pathol, Vol. 107, 3, pp. 321--331, March 1997 Bibtex Bibtex : kiss97 Renard, N, Goldschmidt, D, Decaestecker, C, Lorea, P, Berthe, J V, Verraes, S, Ghanem, G, Salmon, I, Kiss, R, Characterization of the nuclear deoxyribonucleic acid content and nuclear morphometry in 71 primary cutaneous melanomas Dermatology, Vol. 194, 4, pp. 318--324, 1997 Bibtex Bibtex : renard97 Lopes MB, Salmon I, Nagy N, Decaestecker C, Pasteels JL, Laws ER Jr, Kiss R, Computer-Assisted Microscope Analysis of Feulgen-Stained Nuclei in Gonadotroph Adenomas and Null-Cell Adenomas of the Pituitary Gland Endocr Pathol, Vol. 8, 2, pp. 109--120, 1997 Bibtex Bibtex : lopes97 Note : JOURNAL ARTICLE Budel, V, Gasperin, P, Hatschbach, S, Cavalcanti, T C, Petein, M, Decaestecker, C, Darro, F, Salmon, I, Kiss, R, Ploidy level determination and quantitative chromatin pattern description in pregnancy-associated breast cancers Breast Cancer Res Treat, Vol. 45, 2, pp. 109--120, 1997 Bibtex Bibtex : budel97 Petein, M, Decaestecker, C, Segers, V, Janssen, T, Fourmarier, M, Van Leer, P, van Velthoven, R, Schulman, C, Pasteels, J L, Kiss, R, Does any correlation exist between the Gleason classification system and the computer-assisted microscope analysis of Feulgen-stained nuclei features in human prostate adenocarcinoma? Anal Cell Pathol, Vol. 12, 3, pp. 159--171, Dec 1996 Bibtex Bibtex : petein96 Remmelink, M, Salmon, I, Goldschmidt, D, Decaestecker, C, Nemec, E, Berthe, J V, Petein, M, Pasteels, J L, Kiss, R, Quantitative measurements of desmin and vimentin immunostains and cell density in leiomyomas and leiomyosarcomas Anal Cell Pathol, Vol. 12, 1, pp. 25--44, Oct 1996 Bibtex Bibtex : remmelink96a Decaestecker, C, Remmelink, M, Salmon, I, Camby, I, Goldschmidt, D, Petein, M, Van Ham, P, Pasteels, J L, Kiss, R, Methodological aspects of using decision trees to characterise leiomyomatous tumors Cytometry, Vol. 24, 1, pp. 83--92, May 1996 Bibtex Bibtex : decaestecker96a Decaestecker, C, Petein, M, van Velthoven, R, Janssen, T, Raviv, G, Pasteels, J L, Schulman, C, Van Ham, P, Kiss, R, The computer-assisted microscope analysis of Feulgen-stained nuclei linked to a supervised learning algorithm as an aid to prognosis assessment in invasive transitional bladder cell carcinomas Anal Cell Pathol, Vol. 10, 3, pp. 263--280, May 1996 Bibtex Bibtex : decaestecker96b Decaestecker, C, van Velthoven, R, Petein, M, Janssen, T, Salmon, I, Pasteels, J L, Van Ham, P, Schulman, C, Kiss, R, The use of the decision tree technique and image cytometry to characterize aggressiveness in World Health Organization (WHO) grade II superficial transitional cell carcinomas of the bladder J Pathol, Vol. 178, 3, pp. 274--283, March 1996 Bibtex Bibtex : decaestecker96c Remmelink, M, Salmon, I, Goldschmidt, D, Decaestecker, C, Petein, M, Pasteels, J L, Kiss, R, The value of nuclear DNA and texture analysis by digital image processing in the diagnosis of lipomatous and leiomyomatous tumours Anal Cell Pathol, Vol. 10, 1, pp. 45--58, Jan 1996 Bibtex Bibtex : remmelink96b Berthe, J V, Goldschmidt, D, Salmon, I, Decaestecker, C, Remmelink, M, Petein, M, Pasteels, J L, Roels, H, Frierson, H Jr, Kiss, R, Image cytometry analysis of Feulgen-stained nuclei in 72 lipomatous lesions including atypical lipomas and well-differentiated liposarcomas Am J Clin Pathol, Vol. 106, 3, pp. 289--297, 1996 Bibtex Bibtex : berthe96 Goldschmidt, D, Decaestecker, C, Berthe, J V, Gordower, L, Remmelink, M, Danguy, A, Pasteels, J L, Salmon, I, Kiss, R, The contribution of image cytometry and artificial intelligence-related methods of numerical data analysis for adipose tumor histopathologic classification Lab Invest, Vol. 75, 3, pp. 295--306, 1996 Bibtex Bibtex : goldschmidt96 Salmon, I, Rorive, S, Camby, I, Decaestecker, C, Pirotte, B, Rombaut, K, Haot, J, Pasteels, J L, Brotchi, J, Kiss, R, Stereotactic biopsies from astrocytic tumors. Diagnostic information contributed by the quantitative chromatin pattern description Anal Quant Cytol Histol, Vol. 17, 5, pp. 332--343, Oct 1995 Bibtex Bibtex : salmon95 Decaestecker, C, Remmelink, M, Camby, I, Salmon, I, Goldschmidtf, D, Van Ham, P, Pasteels, J L, Kiss, R, The combination of a decision tree technique with the computer-assisted microscope analysis of Feulgen-stained nuclei to assess aggressiveness in lipomatous and smooth muscle tumors Anticancer Res, Vol. 15, 4, pp. 1311--1317, July 1995 Bibtex Bibtex : decaestecker95b Decaestecker, C, Salmon, I, Camby, I, Dewitte, O, Pasteels, J L, Brotchi, J, Van Ham, P, Kiss, R, Identification of high versus lower risk clinical subgroups in a group of adult patients with supratentorial anaplastic astrocytomas J Neuropathol Exp Neurol, Vol. 54, 3, pp. 371--384, May 1995 Bibtex Bibtex : decaestecker95a Poortmans, J R, Brauman, H, Staroukine, M, Verniory, A, Decaestecker, C, Leclercq, R, Indirect evidence of glomerular/tubular mixed-type postexercise proteinuria in healthy humans Am J Physiol, Vol. 254, 2 Pt 2, pp. 277--283, Feb 1988 Bibtex Bibtex : poortmans88 ConferencesMoles Lopez X, Debeir O, Maris C, Roland I, Salmon I, Decaestecker C,Ki-67 hot-spots detection on glioblastoma tissue sections Proceedings of the 2010 IEEE International Symposium on Biomedical Imaging: From Nano to Macro (ISBI 2010), pp. 149-152, 2010 Bibtex Bibtex : moleslopez10 S. Safin , A. Defays , A. Billon, C. Decaestecker, N. Warzée, Influence d'images évocatrices et distractrices sur une tâche de jugement en acoustique des salles. IHM - International Conference Proceeding Series, pp. 241-248, 2009 Bibtex Bibtex : safin09a O. Debeir, I. Adanja, N. Warzée, P. Van Ham, C. Decaestecker, Phase contrast image segmentation by weak watershed transform assembly Proceedings of 5th IEEE International Symposium on Biomedical Imaging : From Nano to Macro (ISBI), pp. 724-727, 2008 Bibtex Bibtex : debeir08d O. Debeir, R. Van Ginckel, I. Adanja, F. Darro, R. Kiss, C. Decaestecker, High throughput characterization of anti-cancer compounds by means of cellular imaging and automatic image analysis. 2007 AACR Annual Meeting in Los Angeles, CA, Apr 2007 Bibtex Bibtex : debeir07 O. Debeir, I. Adanja, R. Kiss, C. Decaestecker, Automatic in vitro cell division characterization by automatic image sequence analysis. Belgian Day on Biomedical Engineering - IEEE Benelux EMBS Symposium. Brussels 7-8 December 2006, Dec 2006 Bibtex Bibtex : debeir06p2 V. Mégalizzi, V. Mathieu, T. Mijatovic, P. Gailly, O. Debeir, M. Van Damme, C. Decaestecker, Y. Kondo, R. Kiss, F. Lefranc, 4-IBP, A SIGMA1 RECEPTOR AGONIST, DECREASES IN VITRO THE MIGRATION OF HUMAN CANCER CELLS, INCLUDING GLIOBLASTOMA CELLS, AND SENSITIZES THEM TO THE CYTOTOXIC INSULTS OF PRO-APOPTOTIC AND PRO-AUTOPHAGIC DRUGS Autumn Meeting 2006 on Cytoskeleton and Cellular Imaging of BSCDB. Ghent, October 14th, Oct 2006 Bibtex Bibtex : megalizzi06p1 O. Debeir, R. Kiss, C. Decaestecker, Cell division characterization by automatic cell tracking and cell lineage reconstruction. Autumn Meeting 2006 on Cytoskeleton and Cellular Imaging of BSCDB. Ghent, October 14th, Oct 2006 Bibtex Bibtex : debeir06p1 O. Debeir, C. Decaestecker, Mitotic Tree Construction by Computer In Vitro Cell Tracking : a Tool for Proliferation and Motility Features Extraction. Proc. of the EUROCON 2005. Serbia and Montenegro, Belgrade, November 22-24, 2005, Nov 2005 Bibtex Bibtex : tree05 C. Hayot, O. Debeir, P. Van Ham, M. Van Damme, R. Kiss, C. Decaestecker, Characterization of the anti-tumor cell migration activities of actin-affecting drugs. CELL MIGRATION IN CANCER AND IMMUNITY Joint Spring Meeting of the Belgian Society for Cell and Developmental Biology, Brussels, May 27-28, 2005, May 2005 Bibtex Bibtex : hayot05b S. Servotte, I. Camby, O. Debeir, C. Deroanne, C-M. Lapière, R. Kiss, B. Nusgens, C. Decaestecker, Neurotensin activates rac1 and cdc42, but not rhoa, in human U373 glioblastoma cells, and modifies their migratory patterns. CELL MIGRATION IN CANCER AND IMMUNITY Joint Spring Meeting of the Belgian Society for Cell and Developmental Biology, Brussels, May 27-28, 2005, May 2005 Bibtex Bibtex : servotte05b O. Debeir, P. Van Ham, R. Kiss, C. Decaestecker, Tracking of migrating cells under phase-contrast video microscopy with combined mean-shift processes. CELL MIGRATION IN CANCER AND IMMUNITY Joint Spring Meeting of the Belgian Society for Cell and Developmental Biology, Brussels, May 27-28, 2005, May 2005 Bibtex Bibtex : debeir05d P. Latinne, O. Debeir, C. Decaestecker, Limiting the Number of Trees in Random Forests. Multiple Classifier Systems, Vol. 2096, pp. 178-187, 2001 Bibtex Bibtex : latinne01limit P. Latinne, O. Debeir, C. Decaestecker, Different Ways of Weakening Decision Trees and Their Impact on Classification Accuracy of DT Combination. Multiple Classifier Systems, Vol. 1857, pp. 200-209, 2000 Bibtex Bibtex : latinne00weak P. Latinne, O. Debeir, C. Decaestecker, Mixing Bagging and Multiple Feature Subsets to Improve Classification Accuracy of Decision Tree Combination. BENELEARN 2000, Tenth Belgian-Dutch Conference on Machine Learning, Tilburg University, Netherlands, December 13 2000., 2000 Bibtex Bibtex : latinne00mixing Books chaptersDebeir O., Adanja I., Kiss R., Decaestecker C.,Models of cancer cell migration and cellular imaging and analysis The Motile Actin System in Health and Disease, pp. 123-156, Kerala (India), 2008 Bibtex Bibtex : debeir08b |